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Membrane Metallo-Endopeptidase (MME) (C-Term), (AA 506-534) antibody

Details for Product No. ABIN391499
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Antigen
Synonyms MME, NEP, CD10, CALLA, CAK, CD167, DDR, EDDR1, HGK2, MCK10, NTRK4, PTK3, PTK3A, RTK6, TRKE, AV378681, Mmel, 6030454K05Rik, C85356, SFE, Nep
Epitope
C-Term, AA 506-534
(17), (17), (14), (8), (4), (4), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(361), (59), (56), (18), (17), (17)
Host
Rabbit
(236), (136), (1)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(34), (33), (31), (14), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)
(198), (138), (84), (72), (56), (51), (36), (28), (13), (11), (4), (3), (2), (1)
Pubmed 4 references available
Quantity 400 µL
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Catalog No. ABIN391499
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Immunogen This Neprilysin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 506-534 AA from the C-terminal region of human Neprilysin.
Clone RB18821
Isotype Ig
Specificity This Neprilysin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 513~542 amino acids from the C-terminal region of human Neprilysin.
Predicted Reactivity Mouse (Murine),Rat (Rattus),Rabbit
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name Neprilysin
Background MME is a common acute lymphocytic leukemia antigen that is an important cell surface marker in the diagnosis of human acute lymphocytic leukemia (ALL). This protein is present on leukemic cells of pre-B phenotype, which represent 85% of cases of ALL. This protein is not restricted to leukemic cells, however, and is found on a variety of normal tissues. It is a oprotein that is particularly abundant in kidney, where it is present on the brush border of proximal tubules and on glomerular epithelium. The protein is a neutral endopeptidase that cleaves peptides at the amino side of hydrophobic residues and inactivates several peptide hormones including glucagon, enkephalins, substance P, neurotensin, oxytocin, and bradykinin.
Synonyms: Neprilysin, Neutral endopeptidase 24.11, Neutral endopeptidase, NEP, Enkephalinase, Atriopeptidase, Common acute lymphocytic leukemia antigen, CALLA, CD_antigen=CD10, EPN, MME
Molecular Weight 85514 DA
Gene ID 4311
UniProt P08473
Research Area Cell Structure
Application Notes WB = 1:1000, IHC = 1:50-100, FACS = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 0.4 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Membrane Metallo-Endopeptidase (MME) (C-Term), (AA 506-534) antibody Western blot analysis of Neprilysin Antibody (C-term) (ABIN391499) in A2058,A375,Ramos cell line lysates (35 µg/lane). EPN (arrow) was detected using the purified polyclonal antibody.
anti-Membrane Metallo-Endopeptidase (MME) (C-Term), (AA 506-534) antibody (2) Formalin-fixed and paraffin-embedded human kidney carcinoma with Neprilysin Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
anti-Membrane Metallo-Endopeptidase (MME) (C-Term), (AA 506-534) antibody (3) Neprilysin Antibody (C-term) (ABIN391499) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Background publications Shipp, Tarr, Chen et al.: "CD10/neutral endopeptidase 24.11 hydrolyzes bombesin-like peptides and regulates the growth of small cell carcinomas of the lung." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 23, pp. 10662-6, 1992 (PubMed).

Shipp, Vijayaraghavan, Schmidt et al.: "Common acute lymphoblastic leukemia antigen (CALLA) is active neutral endopeptidase 24.11 ("enkephalinase"): direct evidence by cDNA transfection analysis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 86, Issue 1, pp. 297-301, 1989 (PubMed).

Wang, Wang, Malter et al.: "Effects of HNE-modification induced by Abeta on neprilysin expression and activity in SH-SY5Y cells." in: Journal of neurochemistry, Vol. 108, Issue 4, pp. 1072-82, 2009 (PubMed).

Dakka, Bellaoui, Bouzid et al.: "CD10 AND CD34 expression in childhood acute lymphoblastic leukemia in Morocco: clinical relevance and outcome." in: Pediatric hematology and oncology, Vol. 26, Issue 4, pp. 216-31, 2009 (PubMed).

Validation Images
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