Images for product: anti-Homeodomain Interacting Protein Kinase 3 (HIPK3) (AA 1127-1156), (C-Term) antibody
Detection of interaction between HIPK3 and SF-1 by coimmunoprecipitation. After expression of SF-1-HA and DYKDDDDK-sHIPK3 (AA 159-1191) in H1299 cells, the HIPK3 protein complex was immunoprecipitated with anti-DYKDDDDK antibody or by direct loading to the gel (input). Western blotting was then performed to detect SF-1-HA and DYKDDDDK-sHIPK3.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma. HC = hepatocarcinoma