Aurora Kinase A (AURKA) (N-Term) antibody

Antigen: Aurora Kinase A (AURKA)

Synonyms: AIK, ARK1, AURA, BTAK, STK6, STK7, STK15, AURORA2, MGC34538, IAK, Ark1, Ayk1, Stk6, AIRK1, AU019385, AW539821, Aurora-A, 87A7-9/2, AurA, ck10, ck[10], l(3)87Ac, l(3)ck10, DmelCG3068, CG3068, MGC124935, aurka, AURKA, MGC134472, aik, ark1, aura, btak, stk6, stk7, stk15, aurora2, zgc:100912

Epitope: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN392389

Additional Information

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Alternative name: AIK (N-term)

Gene ID: 6790

Immunogen: This AIK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human AIK.

Isotype: Ig  (Matching secondary antibodies)

Description: Other names: Aurora/IPL1-related kinase 1, STK6, Aurora-A

Specificity: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human AIK.

Molecular Weight: 46000 DA

Synonyms: AIK, ARK1, AURA, BTAK, STK6, STK7, STK15, AURORA2, MGC34538, IAK, Ark1, Ayk1, Stk6, AIRK1, AU019385, AW539821, Aurora-A, 87A7-9/2, AurA, ck10, ck[10], l(3)87Ac, l(3)ck10, DmelCG3068, CG3068, MGC124935, aurka, AURKA, MGC134472, aik, ark1, aura, btak, stk6, stk7, stk15, aurora2, zgc:100912

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Cycle, Transcription Factors, Signaling, Metabolism, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of AIK (arrow) using rabbit polyclonal hAIK-H105 (ABIN392389). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AIK gene (Lane 2) (Origene Technologies).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with AIK antibody (N-term) (ABIN392389), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.