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PACSIN2 antibody (Protein Kinase C and Casein Kinase Substrate in Neurons 2) (AA 342-371)

Details for Product anti-PACSIN2 Antibody No. ABIN392666, Supplier: Log in to see
Antigen
  • AI197433
  • DKFZp459J1025
  • PACSIN2
  • SdpII
  • sdpii
  • SDPII
  • zgc:77519
Epitope
AA 342-371, C-Term
18
7
4
3
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
48
13
7
5
3
2
2
2
2
1
1
Host
Rabbit
43
5
Clonality (Clone)
Polyclonal ()
Conjugate
This PACSIN2 antibody is un-conjugated
2
2
2
2
2
2
Application
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
43
24
21
13
11
7
1
Supplier
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Immunogen This PACSIN2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 342-371 AA from the C-terminal region of human PACSIN2.
Clone RB3584
Isotype Ig
Specificity This PACSIN2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 349~379 amino acids from the C-terminal region of human PACSIN2.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name PACSIN2 (PACSIN2 Antibody Abstract)
Background PACSIN may play a role in vesicle formation and transport. This protein homo- and hetero-aggregates with other PACSINs. It also binds dynamin 1, synaptojanin, synapsin 1 and the neural Wiskott-Aldrich syndrome protein (N-WASP). The protein exhibits a cvesicle-like cytoplasmic distribution and is ubiquitously expressed. PACSIN is phosphorylated by casein kinase 2 (CK2) and protein kinase C (PKC). The protein contains 1 FCH domain and 1 SH3 domain.
Synonyms: Protein kinase C and casein kinase substrate in neurons protein 2
Molecular Weight 55738 Da
Gene ID 11252
UniProt Q9UNF0
Research Area Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure
Application Notes IF = 1:100, WB = 1:1000, IHC (p) = 1:50-100
Restrictions For Research Use only
Format Liquid
Concentration 0.4 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-PACSIN2 antibody (Protein Kinase C and Casein Kinase Substrate in Neurons 2) (AA 342-371) (ABIN392666) The anti-PACSIN2 Pab (ABIN392666) is used in Western blot to detect PACSIN2 in T-47D ...
Immunohistochemistry (IHC) image for anti-PACSIN2 antibody (Protein Kinase C and Casein Kinase Substrate in Neurons 2) (AA 342-371) (ABIN392666) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary ant...
Product cited in: Cai, Giridharan, Zhang, Saxena, Bahl, Schmidt, Sorgen, Guo, Naslavsky, Caplan: "Differential roles of C-terminal Eps15 homology domain proteins as vesiculators and tubulators of recycling endosomes." in: The Journal of biological chemistry, Vol. 288, Issue 42, pp. 30172-80, 2013 (PubMed).

Giridharan, Cai, Vitale, Naslavsky, Caplan: "Cooperation of MICAL-L1, syndapin2, and phosphatidic acid in tubular recycling endosome biogenesis." in: Molecular biology of the cell, Vol. 24, Issue 11, pp. 1776-90, S1-15, 2013 (PubMed).

de Kreuk, Anthony, Geerts, Hordijk: "The F-BAR protein PACSIN2 regulates epidermal growth factor receptor internalization." in: The Journal of biological chemistry, Vol. 287, Issue 52, pp. 43438-53, 2012 (PubMed).

Hansen, Howard, Nichols: "Pacsin 2 is recruited to caveolae and functions in caveolar biogenesis." in: Journal of cell science, Vol. 124, Issue Pt 16, pp. 2777-85, 2011 (PubMed).

de Kreuk, Nethe, Fernandez-Borja, Anthony, Hensbergen, Deelder, Plomann, Hordijk: "The F-BAR domain protein PACSIN2 associates with Rac1 and regulates cell spreading and migration." in: Journal of cell science, Vol. 124, Issue Pt 14, pp. 2375-88, 2011 (PubMed).

Background publications Strausberg, Feingold, Grouse, Derge, Klausner, Collins, Wagner, Shenmen, Schuler, Altschul, Zeeberg, Buetow, Schaefer, Bhat, Hopkins, Jordan, Moore, Max, Wang, Hsieh, Diatchenko, Marusina, Farmer et al.: "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. ..." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 26, pp. 16899-903, 2002 (PubMed).

Dunham, Shimizu, Roe, Chissoe, Hunt, Collins, Bruskiewich, Beare, Clamp, Smink, Ainscough, Almeida, Babbage, Bagguley, Bailey, Barlow, Bates, Beasley, Bird, Blakey, Bridgeman, Buck, Burgess, Burrill et al.: "The DNA sequence of human chromosome 22. ..." in: Nature, Vol. 402, Issue 6761, pp. 489-95, 1999 (PubMed).