DEFA5 antibody
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- Target See all DEFA5 Antibodies
- DEFA5 (Defensin, alpha 5, Paneth Cell-Specific (DEFA5))
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This DEFA5 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)
- Brand
- IHC-plus™
- Specificity
- This antibody is specific for HD5. It does not cross-react with HD6, lysozyme or sPLA2. Species cross-reactivity: This antibody reacts with human HD5 protein. Other species have not been tested.
- Purification
- Purified
- Immunogen
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Full-length proHD5 protein. [Swiss-Prot# Q01523] The propeptide is the full-length protein. In the mature form, it is cleaved at residue 63 (alanine). Epitope: This antibody has not been epitope mapped.
Type of Immunogen: Purified protein - Isotype
- IgG2b
- Top Product
- Discover our top product DEFA5 Primary Antibody
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- Application Notes
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Approved: IF, IHC, IHC-P (10 μg/mL), WB
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- Tris-glycine, 150 mM sodium chloride, 0.05 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- Target
- DEFA5 (Defensin, alpha 5, Paneth Cell-Specific (DEFA5))
- Alternative Name
- DEFA5 / Defensin 5 (DEFA5 Products)
- Synonyms
- DEF5 antibody, HD-5 antibody, Defa29 antibody, Defcr29 antibody, Defcr5 antibody, DEFA1 antibody, DEFA5 antibody, Defa antibody, defensin alpha 5 antibody, defensin, alpha, 5 antibody, defensin, alpha 5, Paneth cell-specific antibody, alpha-defensin 1A antibody, DEFA5 antibody, Defa5 antibody, MNP1A antibody
- Background
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Name/Gene ID: DEFA5
Synonyms: DEFA5, DEF5, Defensin, alpha 5, Defensin 5, Defensin-5, HD-5, HD5(20-94) - Gene ID
- 1670
- UniProt
- Q01523
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