Biotin antibody (FITC)
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- Target See all Biotin products
- Biotin
- Reactivity
- Please inquire
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Biotin antibody is conjugated to FITC
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Application
- Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Characteristics
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Absorption / Emission: 495 nm / 528 nm
Molar Ratio: 2.7 Moles Fluorescein (FITC) per mole of IgG - Immunogen
- Biotin conjugated Keyhole Limpet Hemocyanin (KLH)
- Isotype
- IgG
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anti-Biotin antibody
WB, ELISA, IP, DB Host: Rabbit Polyclonal unconjugated
anti-Biotin antibodyELISA Host: Goat Polyclonal unconjugated Fab fragment
anti-Biotin antibodyWB, FACS, IHC, IF, ISt, CM Host: Mouse Monoclonal Hyb-8 unconjugated
anti-Biotin antibodyIHC, ISt Host: Rabbit Monoclonal BTN-2032R unconjugated Recombinant Antibody
anti-Biotin antibody (DyLight 488)WB, FM, FLISA Host: Goat Polyclonal DyLight 488
anti-Biotin antibodyFACS, ELISA Host: Mouse Monoclonal SB58c unconjugated
anti-Biotin antibodyIP, Con Host: Mouse Monoclonal unconjugated
anti-Biotin antibodyFACS, ELISA, IP, CyTOF Host: Mouse Monoclonal 1D4-C5 unconjugated
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- Application Notes
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Immunofluorescence. Flow Cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Reconstitution
- Restore with 2.0 mL of deionized water (or equivalent).
- Concentration
- 10.0 mg/mL (by UV absorbance at 280 nm)
- Buffer
- 0.01 M Sodium Phosphate, 0.11 M Sodium Chloride, pH 7.4, 0.01 % (w/v) Thimerisol, 10 mg/mL BSA (IgG and Protease free)
- Preservative
- Thimerosal (Merthiolate)
- Precaution of Use
- This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- Target
- Biotin
- Abstract
- Biotin Products
- Target Type
- Chemical
- Background
- Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.
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