NCS1 antibody

Catalog No. ABIN401204

Product Details anti-NCS1 Antibody

Target: NCS1 (Neuronal Calcium Sensor 1 (NCS1))

Reactivity: Human, Rat, Mouse, Mammalian

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NCS1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Specificity: This antibody was assayed by Immunoblot and found to be reactive against Frequenin at a dilution of 1/5000 followed by reaction with Peroxidase conjugated Affinity Purified Goat anti-Rabbit IgG [H&L] Cat.-No R1364HRP. This antibody is suitable for the detection by Immunoblot of Human, Mouse and Rat Frequenin. Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.

Purification: Immunoaffinity Chromatography using Frequenin (recombinant) coupled to agarose beads.

Immunogen: Frequenin (recombinant from Mouse with extensive post-translational modifications)

Isotype: IgG

Application Details

Application Notes: ELISA. Western Blot: 1/5000. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 2.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide, None

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for NCS1

Target: NCS1 (Neuronal Calcium Sensor 1 (NCS1))

Alternative Name: NCS1 (NCS1 Products)

Synonyms: FREQ antibody, NCS-1 antibody, LOC100189965 antibody, FLUP antibody, freq antibody, freqa antibody, zgc:63620 antibody, 9430075O15Rik antibody, A730032G13Rik antibody, AI836659 antibody, Freq antibody, Mfreq antibody, flup antibody, xfreq antibody, MGC75953 antibody, freqb antibody, wu:fa55b03 antibody, neuronal calcium sensor 1 antibody, neuronal calcium sensor 1a antibody, frequenin homolog antibody, neuronal calcium sensor 1 L homeolog antibody, calcium sensor-1 antibody, calcium-binding protein NCS-1 antibody, Neuronal calcium sensor 1 antibody, neuronal calcium sensor 1b antibody, NCS1 antibody, ncs1a antibody, freq antibody, Ncs1 antibody, ncs1 antibody, ncs1.L antibody, cse-1 antibody, MGYG_03475 antibody, PGTG_09250 antibody, Tsp_06748 antibody, ncs-1 antibody, ncs1b antibody

Background: Frequenin is a Ca2+ binding protein implicated in the regulation of neurotransmitter release at the neuromuscular junction. Frequenin amino acid sequences from different species appear closely related. In mouse a single 4.2 kbp mRNA is produced yielding a protein of 22 kDa when assayed by SDSPAGE under reducing conditions. Frequenin may colocalize with the dendritic marker MAP-2 and is widely distributed in the brain, spinal cord and dorsal root ganglia. Reactivity with avian and invertebrate frequenin is likely. In Drosophila frequenin is related to recoverin and visinin and functions like Ca2+-sensitive guanylyl cyclase activator.Synonyms: FLUP, FREQ, NCS-1, Neuronal calcium sensor 1

Gene ID: 23413

NCBI Accession: NP_001122298

UniProt: P62166