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SMAD3 antibody (SMAD, Mothers Against DPP Homolog 3) (Internal Region)

Details for Product anti-SMAD3 Antibody No. ABIN401376, Supplier: Log in to see
Antigen
  • madh3
  • madh3a
  • smad3
  • wu:fa99e03
  • HSPC193
  • HsT17436
  • JV15-2
  • LDS1C
  • LDS3
  • MADH3
  • XSmad3
  • XenMLP
  • Xmad3
  • madh3-A
  • Madh3
  • AU022421
  • Smad 3
  • mad3
Alternatives
anti-Human SMAD3 antibody for Western Blotting
Epitope
Internal Region
46
46
45
35
28
27
17
16
16
15
13
11
10
9
9
8
7
7
5
5
4
4
4
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
430
200
183
22
18
12
11
10
9
7
6
4
3
2
1
1
1
1
Host
Rabbit
390
51
Clonality
Polyclonal
Conjugate
This SMAD3 antibody is un-conjugated
18
17
15
8
8
8
6
4
4
4
4
4
4
4
4
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
310
209
200
77
67
48
31
19
18
16
8
7
2
2
1
Supplier
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Immunogen Synthetic peptide corresponding to an internal region of human Smad3 protein
Isotype IgG
Specificity This antibody is directed against Smad3 protein.
Cross-Reactivity (Details) Species reactivity (expected):Mouse, Rat.
Species reactivity (tested):Human.
Purification Affinity chromatography
Alternative Name SMAD3 (SMAD3 Antibody Abstract)
Background Smad3 (also known as Mothers against decapentaplegic homolog 3, Mothers against DPP homolog 3, Mad3, hMAD-3, JV15-2 or hSMAD3) is a transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type I receptor kinase. These activators exert diverse effects on a wide array of cellular processes. The Smad proteins mediate much of the signaling responses induced by the TGF-beta superfamily. Briefly, activin type I receptor kinase phosphorylates receptor-activated Smads (R-Smads) at the two extreme serines in the C-terminal SSXS motif, e.g. Smad2 and Smad3 proteins in the TGF-beta pathway, or Smad1, Smad5 or Smad8 in the Bone Morphogenetic Proteins (BMP) pathway. The phosphorylated R-Smad then translocates into the nucleus, where it regulates transcription of target genes. Based on microarray and animal model experiments, Smad3 accounts for at least 80 % of all TGF-beta-mediated responses.Synonyms: JV15-2, MAD homolog 3, MAD-3, MADH3, Mad3, Mothers against DPP homolog 3, Mothers against decapentaplegic homolog 3, SMAD 3, SMAD family member 3, SMAD-3
Gene ID 4088
NCBI Accession NP_001138574
UniProt P84022
Research Area Signaling, Transcription Factors
Pathways Cell Division Cycle
Application Notes ELISA: 1/15,000 - 1/50,000. Western Blot: 1/500 - 1/10,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.06 mg/mL (by UV absorbance at 280 nm)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Supplier Images
 image for anti-SMAD3 antibody (SMAD, Mothers Against DPP Homolog 3) (Internal Region) (ABIN401376) anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (Internal Region) antibody
Background publications Derynck, Zhang: "Smad-dependent and Smad-independent pathways in TGF-beta family signalling." in: Nature, Vol. 425, Issue 6958, pp. 577-84, 2003 (PubMed).

Shi, Massagué: "Mechanisms of TGF-beta signaling from cell membrane to the nucleus." in: Cell, Vol. 113, Issue 6, pp. 685-700, 2003 (PubMed).

Zhang, Feng, We et al.: "Receptor-associated Mad homologues synergize as effectors of the TGF-beta response." in: Nature, Vol. 383, Issue 6596, pp. 168-72, 1996 (PubMed).