SMAD, Mothers Against DPP Homolog 3 (SMAD3) (Internal Region) antibody

Details for Product No. ABIN401376
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Antigen
Synonyms madh3, madh3a, smad3, wu:fa99e03, HSPC193, HsT17436, JV15-2, LDS1C, LDS3, MADH3, XSmad3, XenMLP, Xmad3, madh3-A, Madh3, AU022421, Smad 3, mad3
Epitope
Internal Region
(51), (39), (34), (31), (25), (24), (21), (13), (7), (6), (6), (5), (5), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(344), (201), (192), (65), (52), (41), (15), (12), (4), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Host
Rabbit
(321), (31)
Clonality
Polyclonal
Conjugate
Un-conjugated
(13), (12), (12), (7), (7), (7), (7), (7), (7), (7), (7), (4), (4), (4), (1)
Application
ELISA, Western Blotting (WB)
(238), (162), (121), (82), (70), (39), (33), (11), (9), (9), (3), (2), (2), (1)
Pubmed 3 references available
Quantity 0.1 mg
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Catalog No. ABIN401376
390.50 $
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Immunogen Synthetic peptide corresponding to an internal region of human Smad3 protein
Isotype IgG
Specificity This antibody is directed against Smad3 protein.
Purification Affinity chromatography
Alternative Name SMAD3
Background Smad3 (also known as Mothers against decapentaplegic homolog 3, Mothers against DPP homolog 3, Mad3, hMAD-3, JV15-2 or hSMAD3) is a transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type I receptor kinase. These activators exert diverse effects on a wide array of cellular processes. The Smad proteins mediate much of the signaling responses induced by the TGF-beta superfamily. Briefly, activin type I receptor kinase phosphorylates receptor-activated Smads (R-Smads) at the two extreme serines in the C-terminal SSXS motif, e.g. Smad2 and Smad3 proteins in the TGF-beta pathway, or Smad1, Smad5 or Smad8 in the Bone Morphogenetic Proteins (BMP) pathway. The phosphorylated R-Smad then translocates into the nucleus, where it regulates transcription of target genes. Based on microarray and animal model experiments, Smad3 accounts for at least 80% of all TGF-beta-mediated responses.
Alternate names: JV15-2, MAD homolog 3, MAD-3, MADH3, Mad3, Mothers against DPP homolog 3, Mothers against decapentaplegic homolog 3, SMAD 3, SMAD family member 3, SMAD-3
Gene ID 4088
NCBI Accession NP_001138574
UniProt P84022
Research Area Signaling, Transcription Factors
Application Notes ELISA: 1/15,000 - 1/50,000. Western Blot: 1/500 - 1/10,000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1.06 mg/mL (by UV absorbance at 280 nm)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01% (w/v) Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Expiry Date 12 months
Supplier Images
anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (Internal Region) antibody anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (Internal Region) antibody
Background publications Zhang, Feng, We et al.: "Receptor-associated Mad homologues synergize as effectors of the TGF-beta response." in: Nature, Vol. 383, Issue 6596, pp. 168-72, 1996 (PubMed).

Shi, Massagué: "Mechanisms of TGF-beta signaling from cell membrane to the nucleus." in: Cell, Vol. 113, Issue 6, pp. 685-700, 2003 (PubMed).

Derynck, Zhang: "Smad-dependent and Smad-independent pathways in TGF-beta family signalling." in: Nature, Vol. 425, Issue 6958, pp. 577-84, 2003 (PubMed).

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