NEDD4 antibody (Internal Region)

Catalog No. ABIN401453

Product Details anti-NEDD4 Antibody

Target: NEDD4 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 4, E3 Ubiquitin Protein Ligase (NEDD4))

Binding Specificity: Internal Region

Reactivity: Human, Horse, Macaque, Rabbit, Dog, Chimpanzee

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NEDD4 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: This antibody reacts with endogenous Nedd4 protein.

Cross-Reactivity (Details): Species reactivity (expected):Horse, Macaque, Chimpanzee, Rabbit, Canine, Cattle.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Immunogen: Peptide corresponding to an internal portion of the Nedd4 protein

Isotype: IgG

Application Details

Application Notes: ELISA: 1/20,000 - 1/35,000. Western Blot: 1/300 to 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.64 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for NEDD4

Target: NEDD4 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 4, E3 Ubiquitin Protein Ligase (NEDD4))

Alternative Name: NEDD4 (NEDD4 Products)

Synonyms: NEDD4-1 antibody, RPF1 antibody, AA959633 antibody, AL023035 antibody, AU019897 antibody, E430025J12Rik antibody, Nedd4-1 antibody, Nedd4a antibody, mKIAA0093 antibody, CG32184 antibody, CG42279 antibody, CG7555 antibody, DNedd4 antibody, Dmel\\CG42279 antibody, Dmel_CG32184 antibody, Dmel_CG7555 antibody, dNedd4 antibody, dNedd4-1 antibody, nedd4 antibody, GB16853 antibody, NEDD4 antibody, Nedd4 antibody, neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase antibody, neural precursor cell expressed, developmentally down-regulated 4 antibody, CG42279 gene product from transcript CG42279-RJ antibody, E3 ubiquitin-protein ligase Nedd-4 antibody, E3 ubiquitin-protein ligase NEDD4 antibody, NEDD4 antibody, Nedd4 antibody, LOC411723 antibody, LOC100457150 antibody, nedd4 antibody, LOC100547485 antibody

Background: NEDD4 (neural precursor expressed, developmentally down-regulated protein 4, aliases: KIAA0093, MGC176705, NEDD4-1) is a gene that is highly expressed in early embryonic central nervous system. A family of NEDD4-like proteins has more recently been defined. NEDD4 and NEDD4-like proteins contain multiple functional domains including a calcium-dependent phospholipid and membrane binding domain (C2 domain), two to four protein binding domains (WW domains), and an E3 ubiquitin-protein ligase domain (HECT domain). NEDD4 and NEDD4-2 have been shown to down-regulate both neuronal voltage-gated Na+ channels (NaVs) and epithelial Na+ channels (ENaCs) in response to increased intracellular Na+ concentrations. The WW domains of NEDD4 bind to PY motifs (amino acid sequence PPXY) found in multiple NaV and ENaC proteins, and ubiquitination of these proteins, mediated by the HECT domain of NEDD4, results in their internalization and removal from the plasma membrane. Mutation of the PY motifs in ENaC proteins is associated with Liddle's Syndrome, an autosomal-dominant form of hypertension. In addition to targeting sodium channels, NEDD4-2 has also been shown to negatively regulate TGF-β signaling by targeting Smad2 for degradation. Mouse and human NEDD4 are cleaved by caspase proteins during apoptosis, although the significance of this cleavage is not clear.Synonyms: E3 ubiquitin-protein ligase NEDD4, KIAA0093

Gene ID: 4734

NCBI Accession: NP_006145

UniProt: P46934

Pathways: Notch Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Skeletal Muscle Fiber Development, Signaling Events mediated by VEGFR1 and VEGFR2