TPP1 antibody (Internal Region)

Catalog No. ABIN401515

Product Details anti-TPP1 Antibody

Target: TPP1 (Tripeptidyl Peptidase I (TPP1))

Binding Specificity: Internal Region

Reactivity: Human, Cow, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This TPP1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Sequence: c-TPSVIRKRYN LTSQD

Specificity: This antibody detects Tripeptidyl-peptidase 1 (TPP1) (Internal).

Cross-Reactivity (Details): Species reactivity (expected):Dog, CowSpecies reactivity (tested):Human

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from the internal region of the protein sequence according to NP_000382.3. Genename: TPP1

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1: 4000. Western blot: 0.3-1 μg/mL. Approx 65 kDa band observed in Human Placenta lysates(calculated MW of 61.5 kDa according to NP_000382.3).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Target Details for TPP1

Target: TPP1 (Tripeptidyl Peptidase I (TPP1))

Alternative Name: Tripeptidyl-Peptidase 1 (TPP1) (TPP1 Products)

Synonyms: cln2 antibody, fa01b09 antibody, im:7149243 antibody, wu:fa01b09 antibody, CLN2 antibody, LPIC antibody, TPP-1 antibody, TPP-I antibody, Cln2 antibody, tripeptidyl peptidase I antibody, tripeptidyl-peptidase 1 antibody, tripeptidyl peptidase 1 antibody, tpp1 antibody, NAEGRDRAFT_78259 antibody, MCYG_00184 antibody, MGYG_05881 antibody, MGYG_00757 antibody, TPP1 antibody, Tpp1 antibody

Background: TPP1 (Tripeptidyl peptidase I) is a lysosomal serine protease which cleaves N-terminal tripeptides from substrates. It requires substrates with an unsubstituted N-terminus. TPP1 may act as a non-specific lysosomal peptidase generating tripeptides from the breakdown products produced by lysosomal proteinases. Mutations in this gene result in late-infantile neuronal ceroid lipofuscinosis.Synonyms: Cell growth-inhibiting gene 1 protein, GIG1, LPIC, Lysosomal pepstatin-insensitive protease, TPP-1, TPP-I, Tripeptidyl aminopeptidase

Gene ID: 1200

NCBI Accession: NP_000382

UniProt: O14773

Pathways: Cell Division Cycle, ER-Nucleus Signaling