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JNK antibody (Mitogen-Activated Protein Kinase 8) (pThr183)

Details for Product anti-MAPK8 Antibody No. ABIN401563, Supplier: Log in to see
Antigen
Alternatives
anti-Human JNK antibody for Immunohistochemistry (Frozen Sections)
Epitope
pThr183, pTyr185
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Reactivity
Human, Mouse (Murine), Rat (Rattus)
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Host
Rabbit
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Clonality
Polyclonal
Conjugate
This JNK antibody is un-conjugated
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Application
Immunofluorescence (IF), Western Blotting (WB)
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Supplier
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Immunogen The antiserum was produced against synthesized phosphopeptide derived from human JNK1/JNK2 around the phosphorylation site of Thr183/Tyr185 (M-M-Tp-P-YP-V-V).
Specificity This antibody detects endogenous levels of JNK1/JNK2 only when phosphorylated at Thr183/Tyr185.
Purification Immunoaffinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Alternative Name MAPK8 / JNK1 (MAPK8 Antibody Abstract)
Background JNK (c-Jun N-terminal Kinase), also referred to as Stress Activated Protein Kinase (SAPK), is one of the main mitogen-activated protein kinases (MAPKs) in mammals. JNK is expressed as ten different isoforms due to differential mRNA splicing. The predominant forms are JNK1 and JNK2. JNK is activated by a variety of cellular signals including growth factors, inflammatory cytokines, and environmental stress. The JNK/SAPK signaling pathway involves sequential activation of MAPK kinase kinase (MEKK1), MAPK kinase 4 (MKK4) or MKK7, SAPK/JNK, and c-Jun. Full activation of JNK requires phosphorylation of a threonine and a tyrosine residue in the motif Thr-Pro-Tyr. MKK7 and MKK4 phosphorylate JNK at threonine 183 and tyrosine 185, respectively. The JNK pathway functions to modulate cell cycle, apoptotic and transcriptional responses to stress.Synonyms: JNK-46, JNK-55, JNK1/JNK2, MAPK8, MAPK9, PRKM8, PRKM9, SAPK1, c-Jun N-terminal kinase 1, c-Jun N-terminal kinase 2
Gene ID 5599
NCBI Accession NP_002741
UniProt P45983
Pathways MAPK Signaling, WNT Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway
Application Notes Western blot: 1/500approx. 1/1000. Immunofluorescence: 1/100approx. 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody (in aliquots) at-20 °C.
Supplier Images
 image for anti-JNK antibody (Mitogen-Activated Protein Kinase 8) (pThr183) (ABIN401563) anti-Mitogen-Activated Protein Kinase 8 (MAPK8) (pThr183), (pTyr185) antibody
 image for anti-JNK antibody (Mitogen-Activated Protein Kinase 8) (pThr183) (ABIN401563) anti-Mitogen-Activated Protein Kinase 8 (MAPK8) (pThr183), (pTyr185) antibody (Image 2)
Background publications Kyriakis, Avruch: "Mammalian mitogen-activated protein kinase signal transduction pathways activated by stress and inflammation." in: Physiological reviews, Vol. 81, Issue 2, pp. 807-69, 2001 (PubMed).

Ichijo: "From receptors to stress-activated MAP kinases." in: Oncogene, Vol. 18, Issue 45, pp. 6087-93, 1999 (PubMed).

Davis: "Signal transduction by the c-Jun N-terminal kinase." in: Biochemical Society symposium, Vol. 64, pp. 1-12, 1999 (PubMed).