Cofilin 1 (Non-Muscle) (CFL1) (pTyr140) antibody

Details for Product No. ABIN401587, Supplier: Log in to see
Antigen
  • cfl1
  • xac1
  • xac2
  • cfl
  • cb86
  • sb:cb86
  • zgc:56501
  • wu:fb18a04
  • wu:fk77b03
  • 12/8
  • ADF
  • ADF/cofilin
  • Adf
  • CG4254
  • Cadf
  • D-cof
  • D61
  • Dmel\\CG4254
  • T8
  • Tsr
  • cofilin
  • cofilin/ADF
  • cofilin/actin depolymerizing factor homolog
  • l(2)03101
  • l(2)k03101
  • l(2)k05633
  • l(2)k13409
  • ntf
  • CFL
  • AA959946
  • Cof
  • CFL1
  • cofilin 1 (non-muscle)
  • twinstar
  • Cof1p
  • cofilin
  • cofilin 1 (non-muscle)-like
  • cofilin 1, non-muscle
  • COFILIN protein
  • Contrafreeloading
  • cfl1-a
  • cfl1
  • CFL1
  • tsr
  • COF1
  • TSTA_042790
  • Cfl1
  • LOC100341589
  • COFILIN
  • CFL
Alternatives
anti-Human Cofilin 1 (Non-Muscle) antibody for Immunofluorescence (fixed cells)
Epitope
pTyr140
58
38
28
23
15
14
14
13
11
10
6
5
4
3
3
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
325
198
161
12
11
9
7
5
2
2
1
1
1
1
Host
Rabbit
259
62
4
Clonality
Polyclonal
Conjugate
Un-conjugated
13
13
11
9
8
7
4
4
3
3
3
3
3
3
3
3
3
2
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
230
131
105
69
65
41
40
29
17
7
5
4
4
3
2
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Immunogen The antiserum was produced against synthesized phosphopeptide derived from human cofilin1 around the phosphorylation site of Tyrosine 139 (N-C-Yp-E-E).
Specificity Antibody AP09483PU detects endogenous levels of Cofilin-1 only when phosphorylated at Tyrosine 139.
Purification Immunoaffinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Alternative Name Cofilin-1 (CFL1 Antibody Abstract)
Background Cofilin is a small phosphoinositide sensitive, actin binding protein capable of depolymerizing actin filaments in vitro. Under certain conditions, it fragments the filaments and accelerates actin subunit dissociation from their 'pointed' (minus) ends. Cofilin binds stoichiometrically to monomeric G-actin and to actin protomers in filaments in an apparent pH -dependent, Ca2+- independent manner. Cofilin intercalates between longitudinally associated actin monomers within the filament and distorts its helical twist. Cofilin is ubiquitous in tissues of eukaryotes and is especially abundant in neuronal tissues. It is essential for viability and is important for many cellular processes involving actin remodeling such as motility at the leading edge of cells, polarized cell growth, endocytosis, phagocytosis, cellular activation, cytokinesis, and pathogen intracellular motility.Synonyms: 18 kDa phosphoprotein, CFL, CFL1, Cofilin non-muscle isoform, p18
Gene ID 1072
NCBI Accession NP_005498
UniProt P23528
Pathways Regulation of Cell Size, Tube Formation, Protein targeting to Nucleus, CXCR4-mediated Signaling Events
Application Notes Immunohistochemistry on Paraffin Sections: 1/50approx. 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody (in aliquots) at-20 °C.
Supplier Images
 image for anti-Cofilin 1 (Non-Muscle) (CFL1) (pTyr140) antibody (ABIN401587) anti-Cofilin 1 (Non-Muscle) (CFL1) (pTyr140) antibody
Background publications Chen, Cizeau, Vande Velde, Park, Bozek, Bolton, Shi, Dubik, Greenberg: "Nix and Nip3 form a subfamily of pro-apoptotic mitochondrial proteins." in: The Journal of biological chemistry, Vol. 274, Issue 1, pp. 7-10, 1999 (PubMed).

Yang, Higuchi, Ohashi, Nagata, Wada, Kangawa, Nishida, Mizuno: "Cofilin phosphorylation by LIM-kinase 1 and its role in Rac-mediated actin reorganization." in: Nature, Vol. 393, Issue 6687, pp. 809-12, 1998 (PubMed).

Arber, Barbayannis, Hanser, Schneider, Stanyon, Bernard, Caroni: "Regulation of actin dynamics through phosphorylation of cofilin by LIM-kinase." in: Nature, Vol. 393, Issue 6687, pp. 805-9, 1998 (PubMed).