High Mobility Group Nucleosome Binding Domain 1 (HMGN1) antibody

Antigen: High Mobility Group Nucleosome Binding Domain 1 (HMGN1)

Synonyms: hmg14, hmgn1b, MGC64449, MGC75785, HMG14, FLJ27265, FLJ31471, MGC104230, MGC117425, Hmg14, HMG-14, MGC103397, MGC127465

Clonality: Polyclonal

Host: Rabbit

Reactivity: Cow (Bovine), Human, Rat (Rattus), Mouse (Murine), Dog (Canine), Rabbit

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Catalog no.: ABIN404814

Additional Information

Alternative name: HMGN1

Gene ID: HMGN1

UniProt: P05114

Immunogen: The immunogen for anti-HMGN1 antibody: synthetic peptide directed towards the middle region of human HMGN1

Sequence: PKKAAAKDKSSDKKVQTKGKRGAKGKQAEVANQETKEDLP AENGETKTEE

Cross-Reactivity: Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rabbit, Rat (Rattus)

Format: Lyophilized

Reconstitution: Add 50 µl of distilled water. Final anti-HMGN1 antibody concentration is 1 mg/ml.

Description: Chromosomal protein HMG14 (HMGN1) and its close analog HMG17 (MIM 163910) bind to the inner side of the nucleosomal DNA, potentially altering the interaction between the DNA and the histone octamer. The 2 proteins may be involved in the process that maintains transcribable genes in a unique chromatin conformation. Their ubiquitous distribution and relative abundance, as well as the high evolutionary conservation of the DNA-binding domain of the HMG14 family of proteins, suggest that they may be involved in an important cellular function.Chromosomal protein HMG14 and its close analog HMG17 (MIM 163910) bind to the inner side of the nucleosomal DNA, potentially altering the interaction between the DNA and the histone octamer. The 2 proteins may be involved in the process that maintains transcribable genes in a unique chromatin conformation. Their ubiquitous distribution and relative abundance, as well as the high evolutionary conservation of the DNA-binding domain of the HMG14 family of proteins, suggest that they may be involved in an important cellular function.[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Characteristics: Antigen Size: 100 amino acids

Molecular Weight: 11kDa

Application Details

Application Notes: WB Suggested Anti-HMGN1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: 721_B cell lysate.

Purification: Affinity Purified

Buffer: PBS

Storage (Long Term): For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.

Storage (Short Term): Any unfrozen and/or unused material can be stored at 4°C for short term use (< 1 week), and should not be re-frozen.

Restrictions: For Research Use only

Images

WB Suggested Anti-HMGN1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: 721_B cell lysate

anti-High Mobility Group Nucleosome Binding Domain 1 (HMGN1) antibody (Image 2)

Publications

Product: Zhu, Hansen: "HMGN1 modulates estrogen-mediated transcriptional activation through interactions with specific DNA-binding transcription factors." in: Molecular and cellular biology, Vol. 27, Issue 24, pp. 8859-73, 2007 (PubMed).