Peptidase D (PEPD) (Middle Region) antibody

Details for Product No. ABIN406596
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Antigen
Synonyms MGC89151, DDBDRAFT_0190220, DDBDRAFT_0266378, DDB_0190220, DDB_0266378, PROLIDASE, cb1000, fj78g11, wu:fj78g11, prolidase, Pep-4, Pep4
Epitope
Middle Region
(7), (7), (2), (2), (1), (1), (1)
Reactivity
Mouse (Murine), Rat (Rattus), Cow (Bovine), Zebrafish (Danio rerio), Xenopus laevis, Human, Chicken
(29), (5), (3)
Host
Rabbit
(16), (15)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(28), (16), (6), (6), (5), (4), (4), (2)
Pubmed 1 reference available
Quantity 50 µg
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Catalog No. ABIN406596
289.00 $
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Immunogen Synthetic peptide directed towards the middle region of human PEPD
Sequence LGAVFMPHGL GHFLGIDVHD VGGYPEGVER IDEPGLRSLR TARHLQPGMV
Predicted Reactivity Dog : 100 %, Guinea pig : 100 %, Horse : 100 %, Human : 100 %, Mouse : 100 %, Pig : 100 %, Rat : 100 %, Chicken : 90 %, Rabbit : 90 %, Bovine : 90 %, Zebrafish : 80 %
Characteristics This is a rabbit polyclonal antibody against PEPD. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name PEPD
Background Xaa-Pro dipeptidase is a cytosolic dipeptidase that hydrolyzes dipeptides with proline or hydroxyproline at the carboxy terminus (but not Pro-Pro). It is important in collagen metabolism because of the high levels of iminoacids.Xaa-Pro dipeptidase is a cytosolic dipeptidase that hydrolyzes dipeptides with proline or hydroxyproline at the carboxy terminus (but not Pro-Pro). It is important in collagen metabolism because of the high levels of iminoacids.
Molecular Weight 54 kDa
Gene ID 5184
NCBI Accession NM_000285, NP_000276
UniProt P12955
Research Area Proteolysis / Ubiquitin, Metabolism, Amino Acids
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 493 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
anti-Peptidase D (PEPD) (Middle Region) antibody WB Suggested Anti-PEPD Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: Human Placenta
Product cited in: Surazynski, Donald, Cooper et al.: "Extracellular matrix and HIF-1 signaling: the role of prolidase." in: International journal of cancer. Journal international du cancer, Vol. 122, Issue 6, pp. 1435-40, 2008 (PubMed).

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