SRSF2 antibody (serine/arginine-Rich Splicing Factor 2) (AA 83-113)

Details for Product anti-SRSF2 Antibody No. ABIN453825, Supplier: Log in to see
Antigen
  • PR264
  • SC-35
  • SC35
  • SFRS2
  • SFRS2A
  • SRp30b
  • D11Wsu175e
  • MRF-1
  • Pr264
  • Sfrs10
  • Sfrs2
  • pr264
  • sc-35
  • sc35
  • sfrs2
  • sfrs2a
  • srp30b
  • serine/arginine-rich splicing factor 2
  • SRSF2
  • Srsf2
  • srsf2
Epitope
AA 83-113, Middle Region
5
4
3
3
2
2
2
1
1
1
1
Reactivity
Human
33
12
9
4
3
3
3
3
2
2
2
2
2
Host
Rabbit
28
5
Clonality
Polyclonal
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
28
12
6
6
5
5
5
2
1
1
Options
Supplier
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Immunogen KLH conjugated synthetic peptide selected from aa 83-113 from the Center region of human SFRS2
Specificity This antibody reacts to SFRS2.
Purification Saturated Ammonium Sulfate (SAS) precipitation
Alternative Name SFRS2 / SC-35 (SRSF2 Antibody Abstract)
Background SFRS2 is necessary for the splicing of pre-mRNA. The protein is required for formation of the earliest ATP-dependent splicing complex and interacts with spliceosomal components bound to both the 5'- and 3'-splice sites during spliceosome assembly. It also is required for ATP-dependent interactions of both U1 and U2 snRNPs with pre-mRNA. And it interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. It binds to purine-rich RNA sequences, either 5'-AGSAGAGTA-3' (S=C or G) or 5'-GTTCGAGTA-3' and can bind to beta-globin mRNA and commit it to the splicing pathway.Synonyms: Nuclear Speckle Marker, PR264, Splicing component 35 kDa, Splicing factor SC35, Splicing factor arginine/serine-rich 2
Gene ID 6427
NCBI Accession NP_003007
UniProt Q01130
Application Notes ELISA: 1/1,000. Western blotting: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
Western Blotting (WB) image for anti-serine/arginine-Rich Splicing Factor 2 (SRSF2) (AA 83-113), (Middle Region) antibody (ABIN453825) Western blot analysis of SFRS2 Antibody (Center) in mouse stomach tissue lysates (35u...
Background publications Merdzhanova, Edmond, De Seranno, Van den Broeck, Corcos, Brambilla, Brambilla, Gazzeri, Eymin: "E2F1 controls alternative splicing pattern of genes involved in apoptosis through upregulation of the splicing factor SC35." in: Cell death and differentiation, Vol. 15, Issue 12, pp. 1815-23, 2008 (PubMed).

Solis, Peng, Crawford, Phillips, Patton: "Growth hormone deficiency and splicing fidelity: two serine/arginine-rich proteins, ASF/SF2 and SC35, act antagonistically." in: The Journal of biological chemistry, Vol. 283, Issue 35, pp. 23619-26, 2008 (PubMed).

Donev, Newall, Thome, Sheer: "A role for SC35 and hnRNPA1 in the determination of amyloid precursor protein isoforms." in: Molecular psychiatry, Vol. 12, Issue 7, pp. 681-90, 2007 (PubMed).

Sureau, Soret, Vellard, Crochet, Perbal: "The PR264/c-myb connection: expression of a splicing factor modulated by a nuclear protooncogene." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 24, pp. 11683-7, 1993 (PubMed).

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