anti-HSF1 antibody (Heat Shock Factor Protein 1)

Details for Product anti-HSF1 Antibody No. ABIN453930
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Synonyms hsf1, Hsf, xhsf1, AA960185, HSTF1, HSF1, HSF, HSF1c, hsf1a, hsf1b, zHSF1a, zHSF1b, hstf1
Cow (Bovine), Guinea Pig, Hamster, Human, Monkey, Mouse (Murine), Rabbit, Rat (Rattus)
(417), (277), (137), (67), (63), (62), (60), (59), (2), (2), (1), (1), (1), (1)
(323), (143), (35), (1)
Clonality (Clone)
Monoclonal ()
This HSF1 antibody is un-conjugated
(16), (16), (12), (11), (11), (11), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (4), (4), (4), (4), (4), (4), (4), (4)
Electrophoretic Mobility-Shift Assay (EMSA), Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
(414), (236), (154), (149), (145), (121), (116), (65), (40), (6), (5), (5), (4), (2), (1), (1), (1)
Pubmed 10 references available
Quantity 0.1 mg
Shipping to United States ( )
Availability Will be delivered in 6 to 8 Business Days
Immunogen Purified recombinant HSF1 protein
Clone 10H8
Isotype IgG1
Specificity This antibody detects an ~85 kDa protein in unstressed cell lysates, and an ~95 kDa protein in heat shocked cell lysates, corresponding to the molecular mass of inactive and active forms of HSF1 on SDS-PAGE immunoblots.
Cross-Reactivity (Details) Species reactivity (tested):Human, Mouse, Rat, Bovine, Guinea pig, Hamster, Monkey, Rabbit
Characteristics Synonyms: HSTF1, HSF-1, Heat shock transcription factor 1
Purification Purified
Alternative Name Heat Shock Factor 1 / HSF1 (HSF1 Antibody Abstract)
Background HSF1, or heat shock factor 1, belongs to a family of Heat Shock transcription factors that activate the transcription of genes encoding products required for protein folding, processing, targeting, degradation, and function (2). The up-regulation of HSP (heat shock proteins) expression by stressors is achieved at the level of transcription through a heat shock element (HSE) and a transcription factor (HSF) (3, 4, 5). Most HSFs have highly conserved amino acid sequences. On all HSFs there is a DNA binding domain at the Nterminus. Hydrophobic repeats located adjacent to this binding domain are essential for the formation of active trimers. Towards the C-terminal region another short hydrophobic repeat exists, and is thought to be necessary for suppression of trimerization (6). There are two main heat shock factors, 1 and 2. Mouse HSF1 exists as two isoforms, however in higher eukaryotes HSF1 is found in a diffuse cytoplasmic and nuclear distribution in un-stressed cells. Once exposed to a multitude of stressors, it localizes to discrete nuclear granules within seconds. As it recovers from stress, HSF1 dissipates from these granules to a diffuse nuceloplasmic distribution. HSF2 on the other hand is similar to mouse HSF1, as it exists as two isoforms, the alpha form being more transciptionally active than the smaller beta form (7, 8). Various experiments have suggested that HFS2 may have roles in differentiation and development (9, 10, 11).Synonyms: HSF-1, HSTF1, Heat shock transcription factor 1
Gene ID 3297
UniProt Q00613
Application Notes Western blot (see ref.1). Immunoprecipitation. Immunocytochemistry (see ref.1). ELISA (see ref.1). Gel Mobility Shift Assay (see ref.1).
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4, 0.09 % sodium azide in 50 % glycerol
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C. Avoid repeated freezing andthawing.
Shelf life: one year from despatch.
Expiry Date 12 months
Background publications Morano, Thiele: "Heat shock factor function and regulation in response to cellular stress, growth, and differentiation signals." in: Gene expression, Vol. 7, Issue 4-6, pp. 271-82, 1999 (PubMed).

Jolly, Usson, Morimoto: "Rapid and reversible relocalization of heat shock factor 1 within seconds to nuclear stress granules." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 12, pp. 6769-74, 1999 (PubMed).

Morimoto: "Regulation of the heat shock transcriptional response: cross talk between a family of heat shock factors, molecular chaperones, and negative regulators." in: Genes & development, Vol. 12, Issue 24, pp. 3788-96, 1999 (PubMed).

McMillan, Xiao, Shao et al.: "Targeted disruption of heat shock transcription factor 1 abolishes thermotolerance and protection against heat-inducible apoptosis." in: The Journal of biological chemistry, Vol. 273, Issue 13, pp. 7523-8, 1998 (PubMed).

Cotto, Fox, Morimoto: "HSF1 granules: a novel stress-induced nuclear compartment of human cells." in: Journal of cell science, Vol. 110 ( Pt 23), pp. 2925-34, 1998 (PubMed).

Rallu, Loones, Lallemand et al.: "Function and regulation of heat shock factor 2 during mouse embryogenesis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 6, pp. 2392-7, 1997 (PubMed).

Sarge, Park-Sarge, Kirby et al.: "Expression of heat shock factor 2 in mouse testis: potential role as a regulator of heat-shock protein gene expression during spermatogenesis." in: Biology of reproduction, Vol. 50, Issue 6, pp. 1334-43, 1994 (PubMed).

Murphy, Gorzowski, Sarge et al.: "Characterization of constitutive HSF2 DNA-binding activity in mouse embryonal carcinoma cells." in: Molecular and cellular biology, Vol. 14, Issue 8, pp. 5309-17, 1994 (PubMed).

Fiorenza, Farkas, Dissing et al.: "Complex expression of murine heat shock transcription factors." in: Nucleic acids research, Vol. 23, Issue 3, pp. 467-74, 1995 (PubMed).

Goodson, Park-Sarge, Sarge: "Tissue-dependent expression of heat shock factor 2 isoforms with distinct transcriptional activities." in: Molecular and cellular biology, Vol. 15, Issue 10, pp. 5288-93, 1995 (PubMed).

Catalog No. ABIN453930
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