FLT3 antibody

Catalog No. ABIN457417

This Mouse Monoclonal antibody specifically detects FLT3 in FACS and IP. It exhibits reactivity toward Human.

Quick Overview for FLT3 antibody (ABIN457417)

Target: FLT3 (Fms-Related tyrosine Kinase 3 (FLT3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This FLT3 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP)

Clone: BV10A4

Product Details anti-FLT3 Antibody

Purpose: Anti-Hu CD135 Purified

No Cross-Reactivity: Mouse

Cross-Reactivity (Details): Human

Characteristics: The mouse monoclonal antibody BV10A4 (BV10) reacts with an extracellular epitope of CD135 (FLT3, FLK2, STK-1), a 130-160 kDa type I transmembrane receptor tyrosine kinase that is involved in early steps of hematopoiesis.

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: BV-173 leukemic cell line

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1-4 μg/mL

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

Target Details for FLT3

Target: FLT3 (Fms-Related tyrosine Kinase 3 (FLT3))

Alternative Name: CD135

Background: Fms related tyrosine kinase 3,CD135 / FLT3, also known as FLK2 or STK-1 is a receptor tyrosine kinase that plays important roles in hematopoiesis. After binding of Flt3 ligand (FL), CD135 homodimerizes and stimulates proliferation, differentiation and protects the cell from apoptosis. The loss of CD90 and gain of CD135 expression marks the loss of self-renewal in hematopoietic stem cell population. Detectable CD135 expression appears first at low levels on the surface of primitive multilineage progenitor cells and disappears during defined stages of B-cell development, but is upregulated and maintained during maturation of monocytes. CD135 is also expressed on thymocytes, dendritic cell progenitors and on mature dendritic cells, as well as on various malignant hematopoietic cells.,FLT3, FLK2, STK1

Gene ID: 2322

UniProt: P36888

Pathways: RTK Signaling