Actin antibody

Antigen: Actin

Synonyms: A, Ac5C, Act, Actin, Actin/BAP47, Actin5C, BAP47, M32055, T11, act 5C, act5C, actin-5C, anon-EST:fe2D2, beta-actin/Bap47, cyt5C, l(1)G0009, l(1)G0010, l(1)G0025, l(1)G0079, l(1)G0117, l(1)G0177, l(1)G0245, l(1)G0330, l(1)G0420, l(1)G0486, DmelCG4027, CG4027, Act57A, Act57b, BEST:RE38067, C, CG30294, M, T10, act 57A, anon-EST:CL2c12, DmelCG10067, CG10067, 143060_f_at, D, act 79B, act79B, mus79B, DmelCG7478, CG7478, Act(88F), Act88-F, E, Ifm(3)7, act 88F, act88F, rsd, DmelCG5178, CG5178, act 87E, act87E, mus87E, DmelCG18290, CG18290, ACTIN, ACTIN-12, actin, dsim_GLEANR_2829, DsimGD19025, GD19025, Act88F, AFFX-Dros-ACTIN_M_r_at, Act42a, act 42A, DmelCG12051, CG12051

Clonality: Monoclonal (HHF35)

Host: Mouse

Reactivity: Human, Primates, Mouse (Murine), Rat (Rattus), Dog (Canine), Cat (Feline), Rabbit, Chicken

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Radioimmunoassay (RIA)

Catalog no.: ABIN457424

Additional Information

Immunogen: SDS extracted protein fraction of human myocardium

Format: Purified

Isotype: IgG1  (Matching secondary antibodies)

Clone: HHF35

Description: Actin is a highly conserved ubiquitous globular protein (G-actin) that polymerizes to formfibrous F-actin microfilaments. In higher eucaryotes several actin isoforms have beenidentified, that fall into three classes. Alpha actin is a structural component of the contractileapparatus of muscle cells or muscle-derived cells. Beta actin and gamma actin play roles inregulation of cell motility in other cell types. Specific subcellular structures such as as stressfibers, focal adhesions, filopodia etc., are formed by involvement of actin cytoskeleton.

Specificity: The mouse monoclonal antibody HHF35 recognizes muscle-specific alpha and gamma actin(42 kDa) in various species. This antibody stains skeletal, smooth and myocardial cells aswell as myoepithelial cells and pericytes of small vessels. It is a widely used marker ofmuscle and muscle-derived cells.

Synonyms: A, Ac5C, Act, Actin, Actin/BAP47, Actin5C, BAP47, M32055, T11, act 5C, act5C, actin-5C, anon-EST:fe2D2, beta-actin/Bap47, cyt5C, l(1)G0009, l(1)G0010, l(1)G0025, l(1)G0079, l(1)G0117, l(1)G0177, l(1)G0245, l(1)G0330, l(1)G0420, l(1)G0486, DmelCG4027, CG4027, Act57A, Act57b, BEST:RE38067, C, CG30294, M, T10, act 57A, anon-EST:CL2c12, DmelCG10067, CG10067, 143060_f_at, D, act 79B, act79B, mus79B, DmelCG7478, CG7478, Act(88F), Act88-F, E, Ifm(3)7, act 88F, act88F, rsd, DmelCG5178, CG5178, act 87E, act87E, mus87E, DmelCG18290, CG18290, ACTIN, ACTIN-12, actin, dsim_GLEANR_2829, DsimGD19025, GD19025, Act88F, AFFX-Dros-ACTIN_M_r_at, Act42a, act 42A, DmelCG12051, CG12051

Application Details

Application Notes: Western Blotting - Recommended dilution: 1 µg/ml. Positive control: murine femoral muscle, murine heart. Negative control: HUVEC line. Application note: reducing conditions. Immunohistochemistry (paraffin sections). Application note: Antigen retrieval steps generally not required, but e. g. in case of arterialsmooth muscle cells or myoepithelial cells, pepsin or trypsin pretreatment is recommended.

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified from cell culture supernatant by protein-A affinity chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Research Area: Cancer, Apoptosis/Necrosis, Cytoskeleton, Microfilaments, Extracellular Matrix

Restrictions: For Research Use only