Actin antibody (C-Term)

Catalog No. ABIN658563

Cited in 2+ publications. The Rabbit Polyclonal anti-Actin antibody (Clone RB35391) (ABIN658563) specifically detects Actin in WB, IHC (p), IF and FACS. The antibody is reactive with Human samples.

Quick Overview for Actin antibody (C-Term) (ABIN658563)

Target: Actin (ACTA1) (Actin, alpha 1, Skeletal Muscle (ACTA1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Actin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: RB35391

Product Details anti-Actin Antibody

Binding Specificity: AA 346-375, C-Term

Specificity: This ACTA1/?-actin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 345-376 amino acids from the C-terminal region of human ACTA1/?-actin.

Predicted Reactivity: Cow (Bovine),Chicken,Mouse (Murine),Pig (Porcine),Rat (Rattus),Rabbit,Xenopus laevis

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This ACTA1/Alpha-actin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 346-375 AA from the C-terminal region of human ACTA1/Alpha-actin.

Isotype: Ig Fraction

Application Details

Application Notes: WB = 1:1000, IHC (p) = 1:10-50, IF = 1:10-50, FACS = 1:10-50

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-Actin antibody (ABIN658563)

Xiao, Gao, Gao, Zhao, Hong, Li, Yao, Cheng, Zhou: "Directed Differentiation of Zebrafish Pluripotent Embryonic Cells to Functional Cardiomyocytes." in: Stem cell reports, Vol. 7, Issue 3, pp. 370-382, (2017) (PubMed).

Ying, Xie, Bai, Chen, Wang, Zhang, Morishita, Sun, Rajagopalan: "Exposure to concentrated ambient particulate matter induces reversible increase of heart weight in spontaneously hypertensive rats." in: Particle and fibre toxicology, Vol. 12, Issue 1, pp. 15, (2015) (PubMed).

Target Details for Actin

Target: Actin (ACTA1) (Actin, alpha 1, Skeletal Muscle (ACTA1))

Background: The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects.
Synonyms: Actin, alpha skeletal muscle,ACTA1,ACTA

Molecular Weight: 42051 Da

Gene ID: 58

UniProt: P68133

Pathways: Caspase Cascade in Apoptosis, Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development