Actin antibody

Catalog No. ABIN4905845

The Mouse Monoclonal anti-Actin antibody is suitable to detect Actin in samples from Human. It has been validated for WB, IHC (p) and FACS.

Quick Overview for Actin antibody (ABIN4905845)

Target: Actin (ACTA1) (Actin, alpha 1, Skeletal Muscle (ACTA1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Actin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Clone: 7C1

Product Details anti-Actin Antibody

Cross-Reactivity: Human

Purification: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Immunogen: This ACTA1 monoclonal antibody is generated from mouse immunized with ACTA1 recombinant protein.

Isotype: IgG1

Application Details

Application Notes: WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for Actin

Target: Actin (ACTA1) (Actin, alpha 1, Skeletal Muscle (ACTA1))

Alternative Name: ACTA1

Background:

Synonyms: ACTA, ASMA, CFTD, MPFD, NEM1, NEM2, NEM3, SHPM, CFTD1, CFTDM, Actin, alpha skeletal muscle, Alpha-actin-1, ACTA1

Background: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Gene ID: 58

UniProt: P68133

Pathways: Caspase Cascade in Apoptosis, Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development