Actin antibody (C-Term)

Catalog No. ABIN3043519

This Rabbit Polyclonal antibody specifically detects Actin in WB and IHC. It exhibits reactivity toward Human, Mouse and Rat. It has been mentioned in 64+ publications

Quick Overview for Actin antibody (C-Term) (ABIN3043519)

Target: Actin (ACTA1) (Actin, alpha 1, Skeletal Muscle (ACTA1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Actin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Actin Antibody

Binding Specificity: AA 277-308, C-Term

Purpose: Anti-Actin/ACTA1 Antibody

Sequence: HETTYNSIMK CDIDIRKDLY ANNVMSGGTT MY

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Actin/ACTA1 Antibody (ABIN3043519). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Actin, identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-Actin antibody (ABIN3043519)

Cui, Wang, Yu, Li, Xie, Gao, Wang, Zhu: "Jia-Shen decoction-medicated serum inhibits angiotensin-II induced cardiac fibroblast proliferation via the TGF-β1/Smad signaling pathway." in: Molecular medicine reports, Vol. 14, Issue 2, pp. 1610-6, (2017) (PubMed).

Yan, Chen, Gong, Yin, Zhou, Chaugai, Wang: "miR-21-3p regulates cardiac hypertrophic response by targeting histone deacetylase-8." in: Cardiovascular research, Vol. 105, Issue 3, pp. 340-52, (2015) (PubMed).

Mitsuhashi, Shindo, Shigetomi, Miyamoto, Ubukata: "(+)-Epogymnolactam, a novel autophagy inducer from mycelial culture of Gymnopus sp." in: Phytochemistry, Vol. 114, pp. 163-7, (2015) (PubMed).

Wu, Sun, Wu, Li, Du, Zeng, Bi, Sun: "Activation of calcium-sensing receptor increases TRPC3/6 expression in T lymphocyte in sepsis." in: Molecular immunology, Vol. 64, Issue 1, pp. 18-25, (2015) (PubMed).

Hong, Zhang, Zhang, Yang, Wan, Yu, Wang, Feng, Li, Yew: "Expression of dopamine transporter in the different cerebral regions of methamphetamine-dependent rats." in: Human & experimental toxicology, Vol. 34, Issue 7, pp. 707-17, (2015) (PubMed).

Xie, Zhang, Chen, Pan, Wu, Ge, Yang: "Effects of ghrelin on postresuscitation brain injury in a rat model of cardiac arrest." in: Shock (Augusta, Ga.), Vol. 43, Issue 5, pp. 490-6, (2015) (PubMed).

Dai, Yin, Zhao, Guo, Li, Ma, Lu: "III-10, a newly synthesized flavonoid, induces cell apoptosis with the involvement of reactive oxygen species-mitochondria pathway in human hepatocellular carcinoma cells." in: European journal of pharmacology, Vol. 764, pp. 353-62, (2015) (PubMed).

Cheng, Ji, Liu, Mei, Wang, Feng, Ding: "Erratum: Expression changes of ionic channels in early phase of cultured rat atrial myocytes induced by rapid pacing." in: Journal of cardiothoracic surgery, Vol. 10, pp. 10, (2015) (PubMed).

Liu, Wang, Wang, Wang: "Anticancer effects of chemokine receptor 4(CXCR4) gene silenced by CXCR4-siRNA in nude mice model of ovarian cancer." in: Cell biochemistry and biophysics, Vol. 70, Issue 3, pp. 1893-900, (2014) (PubMed).

Chen, Yang, Pang, Peng, Dong, Liu, Ouyang, Ren: "Expression, purification and antibody preparation using different constructs of PCV2 capsid protein." in: International journal of biological macromolecules, Vol. 67, pp. 289-94, (2014) (PubMed).

Gong, Liu, Ding, Liu, Li, Zheng, Jia, Zhou, Zou, Li, Huang, Fan: "Physiological pulsatile flow culture conditions to generate functional endothelium on a sulfated silk fibroin nanofibrous scaffold." in: Biomaterials, Vol. 35, Issue 17, pp. 4782-91, (2014) (PubMed).

Tao, Fu, Wang, Yao, Zhou, Dai, Li, Lu, Wang: "LL-202, a newly synthesized flavonoid, inhibits tumor growth via inducing G(2)/M phase arrest and cell apoptosis in MCF-7 human breast cancer cells in vitro and in vivo." in: Toxicology letters, Vol. 228, Issue 1, pp. 1-12, (2014) (PubMed).

Li, Chen, Ouyang, Chen, Wu, Kuang, Zheng: "Increased autophagy sustains the survival and pro-tumourigenic effects of neutrophils in human hepatocellular carcinoma." in: Journal of hepatology, Vol. 62, Issue 1, pp. 131-9, (2014) (PubMed).

Zhou, Wang, Tokunaga, Huang, Sun, Yang: "The HIV-1 accessory protein Vpr induces the degradation of the anti-HIV-1 agent APOBEC3G through a VprBP-mediated proteasomal pathway." in: Virus research, Vol. 195, pp. 25-34, (2014) (PubMed).

Tang, Liu, Yang, Xie, Ye, Wu, Liu, Chen, Zhang, Xie: "miR-185 suppresses tumor proliferation by directly targeting E2F6 and DNMT1 and indirectly upregulating BRCA1 in triple-negative breast cancer." in: Molecular cancer therapeutics, Vol. 13, Issue 12, pp. 3185-97, (2014) (PubMed).

Cheng, Zhao, Wang, Lu, Wang, Wang, Yao: "The effect of 5'-adenylic acid on hepatic proteome of mice radiated by 60Co γ-ray." in: International journal of molecular sciences, Vol. 15, Issue 1, pp. 186-202, (2014) (PubMed).

Li, Chen, Li, Zhang, Tang: "Cytotoxicity of polyaniline nanomaterial on rat celiac macrophages in vitro." in: PLoS ONE, Vol. 9, Issue 9, pp. e107361, (2014) (PubMed).

Li, Shang: "Inhaled corticosteroids inhibit substance P receptor expression in asthmatic rat airway smooth muscle cells." in: BMC pulmonary medicine, Vol. 12, pp. 79, (2013) (PubMed).

Yu, Dai, Chen, Zang, Deng, Liu, Ying: "Hypolipidemic and antioxidant activities of polysaccharides from Rosae Laevigatae Fructus in rats." in: Carbohydrate polymers, Vol. 94, Issue 1, pp. 56-62, (2013) (PubMed).

Leng, Wang, Pang, Zhan, Zhang, Wang, Gao, Qian: "Evidence of a role for both anti-Hsp70 antibody and endothelial surface membrane Hsp70 in atherosclerosis." in: Cell stress & chaperones, Vol. 18, Issue 4, pp. 483-93, (2013) (PubMed).

Target Details for Actin

Target: Actin (ACTA1) (Actin, alpha 1, Skeletal Muscle (ACTA1))

Alternative Name: ACTA1

Background:

Synonyms: Actin, alpha skeletal muscle,Alpha-actin-1,ACTA1,ACTA,

Tissue Specificity: Detected in serum and cerebrospinal fluid (at protein level). Highly expressed in the choroid plexus. Detected at lower levels in the liver. .

Background: Actin, a highly conserved protein, is a major component of both the cytoskeletal and contractile structures in the cell types. It varies in amount, being related to the type of differentiation and to the functional state of cells and tissues. The actins exhibit over 90 % sequence homology, but each isoform has a unique NH2-terminal sequence. The isoforms are comprised of three alpha-actin, one beta-actin, two gamma-actin. Because the amino acid sequence of the C-terminal is the same for almost all actins, this antibody has been raised using a synthetic peptide corresponding to the C-terminal 11 residues.

Molecular Weight: 42 kDa

Gene ID: 58

UniProt: P68133

Pathways: Caspase Cascade in Apoptosis, Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development