CD44 (CD44) antibody
| Antigen |
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| Synonyms |
IN, LHR, MC56, MDU2, MDU3, MIC4, Pgp1, CDW44, CSPG8, HCELL, MUTCH-I, ECMR-III, MGC10468 |
| Clonality |
Monoclonal (IM7) |
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Host
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Reactivity
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 Alternatives Human, Mouse (Murine), Dog (Canine), Horse (Equine), Cat (Feline)
Human (346), Mouse (Murine) (194), Rat (Rattus) (115), Dog (Canine) (62), Pig (Porcine) (52), Rabbit (51), Cow (Bovine) (35), Chicken (33), Horse (Equine) (27), Monkey (13), Cat (Feline) (12), Sheep (Ovine) (8), Baboon (5), Bird (Avian) (4), Goat (2)
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Application
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Alternatives Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)
Flow Cytometry (FACS) (411), Western Blotting (WB) (131), Immunohistochemistry (Frozen Sections) (IHC (fro)) (119), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (118), Immunoprecipitation (IP) (108), Immunofluorescence (IF) (107), ELISA (52), Immunohistochemistry (IHC) (29), Enzyme Immunoassay (EIA) (11), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (8), Functional Studies (Func) (7), Immunocytochemistry (ICC) (7), Cytotoxicity Test (CyTox) (6), Blocking Antibody (Inhibition) (5), Immunoelectron Microscopy (IEM) (5), ELISA (Detection) (2), Radioimmunoassay (RIA) (2), Blocking Antibody (Inhibition) (1), Immunohistochemistry (Paraformaldehyde) (IHC (pfa)) (1), Neutralization (Neut) (1)
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| Catalog no. |
ABIN457426 |
| Quantity |
0.1 mg (1 mg/ml) |
| Price |
195.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 3 to 5 Business Days |
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Alternative name
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CD44
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Immunogen
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Dexamethasone-induced cells of the SJL mouse spontaneous myeloid leukemia M1
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Format
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Purified
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Isotype
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IgG2b (Matching secondary antibodies)
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Clone
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IM7
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Description
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CD44 is a transmembrane glycoprotein expressed on the surface of most cells, whichserves as a receptor for hyaluronan. CD44 mediates angiogenesis, cell adhesion,proliferation and migration, it is thus important for lymphocyte activation, recirculation andhoming. Although CD44 functions are essential for physiological activities of normal cells,elevated CD44 expression correlates with poor prognosis in many carcinomas, facilitatingtumour growth and metastasis, antiapoptosis and directional motility of cancer cells.
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Specificity
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The rat monoclonal antibody IM7 reacts with CD44 antigen (Phagocyte glycoprotein 1), an80-95 kDa transmembrane glycoprotein (hyaladherin family) present on the most of cellsand tissues (leukocytes, endothelial cells, mesenchymal cells, etc.), it is negative onplatelets and hepatocytes. The antibody reacts with all isoforms of mouse CD44.
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Application Notes
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Flow Cytometry: Recommended dilution: 1 µg/ml. Positive control: mouse peripheral blood. Immunohistochemistry (paraffin sections) - Positive tissue: mouse spleen
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Concentration
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1 mg/ml
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Purity
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> 95% (by SDS-PAGE)
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Purification
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Purified from hybridoma culture supernatant by protein-G affinity chromatography.
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Buffer
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Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
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Storage
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Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.
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Research Area
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Stem Cells, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells, Cancer, Cell Cycle
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Restrictions
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For Research Use only
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Alternatives for antigen "CD44 (CD44)", type "Antibodies"
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Hosts
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Mouse (295), Rabbit (130), Rat (105), Goat (2), Human (1)
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Reactivities
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Human (346), Mouse (Murine) (194), Rat (Rattus) (115), Dog (Canine) (62), Pig (Porcine) (52), Rabbit (51), Cow (Bovine) (35), Chicken (33), Horse (Equine) (27), Monkey (13), Cat (Feline) (12), Sheep (Ovine) (8), Baboon (5), Bird (Avian) (4), Goat (2)
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Applications
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Flow Cytometry (FACS) (411), Western Blotting (WB) (131), Immunohistochemistry (Frozen Sections) (IHC (fro)) (119), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (118), Immunoprecipitation (IP) (108), Immunofluorescence (IF) (107), ELISA (52), Immunohistochemistry (IHC) (29), Enzyme Immunoassay (EIA) (11), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (8), Functional Studies (Func) (7), Immunocytochemistry (ICC) (7), Cytotoxicity Test (CyTox) (6), Blocking Antibody (Inhibition) (5), Immunoelectron Microscopy (IEM) (5), ELISA (Detection) (2), Radioimmunoassay (RIA) (2), Blocking Antibody (Inhibition) (1), Immunohistochemistry (Paraformaldehyde) (IHC (pfa)) (1), Neutralization (Neut) (1)
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Conjugates
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FITC (92), Biotin (48), PE (38), APC (15), RPE (9), Cy5.5 (7), PE,Cy7 (7), Alexa Fluor 488 (6), Alexa Flour 488 (5), Alexa Fluor 350 (5), Alexa Fluor 555 (5), Alexa Fluor 647 (5), Cy3 (5), Cy5 (5), Cy7 (5), Gold (5), HRP (5), PE,Cy3 (5), PE,Cy5 (5), PE,Cy5.5 (5), RBITC (5), Cy5,PE (3), Cy5.5,PE (3), SPRD (3), Cy7,PE (2), Alexa Fluor 700 (1), Pacific Blue (1)
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Epitopes
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pSer706 (1)
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