TGFB1I1 antibody (N-Term)
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- Target See all TGFB1I1 Antibodies
- TGFB1I1 (Transforming Growth Factor beta 1 Induced Transcript 1 (TGFB1I1))
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Binding Specificity
- N-Term
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Reactivity
- Human, Mouse, Rat, Dog, Rabbit
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This TGFB1I1 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Brand
- IHC-plus™
- Specificity
- Human TGFB1I1 / HIC-5
- Predicted Reactivity
- Percent identity by BLAST analysis: Mouse, Rat, Dog, Rabbit (100%) Horse (92%).
- Purification
- Immunoaffinity purified
- Immunogen
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Synthetic peptide from N-Terminus of human TGFB1I1 (B2R8D5, NP_057011). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Mouse, Rat, Panda, Dog, Rabbit (100%), Marmoset, Horse (92%), Bat (84%).
Type of Immunogen: Synthetic peptide - Isotype
- IgG
- Top Product
- Discover our top product TGFB1I1 Primary Antibody
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- Application Notes
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Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. Tested in Western blot using transfected cells. ELISA titer in peptide based assay: 1:312500. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Distilled water
- Concentration
- Lot specific
- Buffer
- Lyophilized from PBS with 2 % sucrose
- Handling Advice
- Avoid repeat freeze-thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
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Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
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- Target
- TGFB1I1 (Transforming Growth Factor beta 1 Induced Transcript 1 (TGFB1I1))
- Alternative Name
- ARA55 / HIC-5 (TGFB1I1 Products)
- Synonyms
- TGFB1I1 antibody, hic5 antibody, ara55 antibody, hic-5 antibody, tsc-5 antibody, ARA55 antibody, HIC-5 antibody, HIC5 antibody, TSC-5 antibody, Hic5 antibody, Ara55 antibody, Hic-5 antibody, ara-55 antibody, transforming growth factor beta 1 induced transcript 1 antibody, transforming growth factor beta 1 induced transcript 1 L homeolog antibody, TGFB1I1 antibody, tgfb1i1 antibody, Tgfb1i1 antibody, tgfb1i1.L antibody
- Background
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Name/Gene ID: TGFB1I1
Family: TGF beta
Synonyms: TGFB1I1, ARA55, HIC5, HIC-5, TSC-5 - Gene ID
- 7041
- NCBI Accession
- NP_057011
- UniProt
- O43294
- Pathways
- Intracellular Steroid Hormone Receptor Signaling Pathway, VEGF Signaling
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