eIF4EBP1 antibody (AA 101-118)

Catalog No. ABIN462434

The Rabbit Polyclonal anti-eIF4EBP1 antibody is suitable to detect eIF4EBP1 in samples from Human, Mouse, Rat, Rabbit and Hamster. It has been validated for WB, IHC, IHC (p) and EMSA.

Quick Overview for eIF4EBP1 antibody (AA 101-118) (ABIN462434)

Target: eIF4EBP1 (EIF4EBP1) (Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1))

Reactivity: Human, Mouse, Rat, Rabbit, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This eIF4EBP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Electrophoretic Mobility-Shift Assay (EMSA)

Product Details anti-eIF4EBP1 Antibody

Binding Specificity: AA 101-118

Specificity: Detects multiple bands at 19-25 kD, corresponding to the apparent molecular mass of the various phosphorylation states of PHAS-I protein on SDS-PAGE immunoblots. This antibody provides a simple read-out for PKB (Akt) activity in vivo through the use of gel-shift assays. Antibody specificity has been confirmed in Western blot peptide competition studies.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Mouse, Rat, Hamster, Rabbit (100%) Marmoset, Goat, Elephant, Dog, Bat, Bovine, Horse (94%) Panda (83%).

Purification: Immunoaffinity purified

Immunogen: An 18 residue synthetic peptide SPEDKRAGGEESQFEMDI based on the human PHAS-I (residues 101-118) and the peptide coupled to KLH. This sequence is identical to rat and mouse PHAS- I over these residues. Percent identity by BLAST analysis: Human, Gorilla, Mouse, Rat, Hamster, Rabbit (100%), Marmoset, Goat, Elephant, Dog, Bat, Bovine, Horse (94%), Panda (83%).

Type of Immunogen: Synthetic peptide - KLH conjugated

Application Details

Application Notes: Approved: GS, IHC, IHC-P (5 - 10 μg/mL), WB (4 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: BBS,  pH 8.4 (25 mM sodium borate, 100 mM boric acid, 75 mM sodium chloride, 5 mM EDTA)

Handling Advice: avoid freeze thaw cycles. For maximum product recovery, after thawing, centrifuge the product vial before removing cap.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. For maximum product recovery, after thawing, centrifuge the product vial before removing cap.

Target Details for eIF4EBP1

Target: eIF4EBP1 (EIF4EBP1) (Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1))

Alternative Name: EIF4EBP1 / 4EBP1

Background: Name/Gene ID: EIF4EBP1

Synonyms: EIF4EBP1, 4E-BP1, 4EBP1, BP-1, PHAS-I, EIF4E-binding protein 1

Gene ID: 1978

UniProt: Q13541

Pathways: MAPK Signaling, PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Regulation of Cell Size, BCR Signaling