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Antibodies
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anti-Lymphocyte-Specific Protein tyrosine Kinase (LCK) (pTyr505) antibody
Lymphocyte-Specific Protein tyrosine Kinase (LCK) (pTyr505) antibody
| Antigen |
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| Synonyms |
YT16, p56lck, pp58lck, Lck1, Lcktkr, MGC136695, MGC194004, MGC194043, zgc:136695, LCK, MGC126900 |
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Epitope
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| Clonality |
Polyclonal |
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Host
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Reactivity
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Application
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 Alternatives Western Blotting (WB), Immunohistochemistry (IHC), ELISA
Western Blotting (WB) (74), Immunoprecipitation (IP) (26), Immunofluorescence (IF) (19), Flow Cytometry (FACS) (18), ELISA (14), Enzyme Immunoassay (EIA) (11), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (8), Immunohistochemistry (IHC) (7), Immunohistochemistry (Frozen Sections) (IHC (fro)) (6), Functional Studies (Func) (5), Immunocytochemistry (ICC) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), ELISA (Detection) (1), Immunoelectron Microscopy (IEM) (1)
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| Catalog no. |
ABIN481947 |
| Quantity |
50µg (1 mg/ml) |
| Price |
150.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Alternative name
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Lck (Phospho-Tyr505)
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Gene ID
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3932 (NCBI Gene Symbol: LCK)
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Swiss-Prot
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P06239
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Immunogen
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The antiserum was produced against synthesized peptide derived from human Lck around the phosphorylation site of Tyr505.
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Format
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Liquid
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Isotype
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IgG (Matching secondary antibodies)
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Description
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Synonyms: kinase Lck, LCK, LSK, LSK-T, lymphocyte-specific protein tyrosine kinase, P56-LCK, T cell-specific protein-tyrosine kinase
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Specificity
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Lck (Phospho-Tyr505) Antibody detects endogenous levels of Lck only when phosphorylated at tyrosine505.
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Molecular Weight
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58 kDa
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Comments
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OMIM-Number: 153390, Unigene-Number: Hs.470627
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Application Notes
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WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:60000
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Concentration
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1 mg/ml
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Purity
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Greater 95%
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Purification
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The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
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Buffer
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Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage
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Store at -20°C/1 year
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Restrictions
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For Research Use only
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Alternatives for antigen "Lymphocyte-Specific Protein tyrosine Kinase (LCK)", type "Antibodies"
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Hosts
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Rabbit (57), Mouse (29)
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Reactivities
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Human (74), Mouse (Murine) (41), Rat (Rattus) (29), Chicken (2)
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Applications
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Western Blotting (WB) (74), Immunoprecipitation (IP) (26), Immunofluorescence (IF) (19), Flow Cytometry (FACS) (18), ELISA (14), Enzyme Immunoassay (EIA) (11), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (8), Immunohistochemistry (IHC) (7), Immunohistochemistry (Frozen Sections) (IHC (fro)) (6), Functional Studies (Func) (5), Immunocytochemistry (ICC) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), ELISA (Detection) (1), Immunoelectron Microscopy (IEM) (1)
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Conjugates
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Alexa Flour 488 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1), RBITC (1)
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Epitopes
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pTyr505 (20), pTyr394 (4), C-Term (3), pTyr393 (3), N-Term (1), phospho (1)
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