ARAF antibody (pTyr302)

Catalog No. ABIN482099

The Rabbit Polyclonal anti-ARAF antibody is suitable to detect ARAF in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IHC and IF.

Quick Overview for ARAF antibody (pTyr302) (ABIN482099)

Target: ARAF (V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARAF antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-ARAF Antibody

Binding Specificity: AA 276-325, pTyr302

Specificity:

A-RAF (Phospho-Tyr302) Antibody detects endogenous levels of A-RAF only when phosphorylated at Tyr302.

PhosphorylationH:Y302 M:Y300 R:Y300

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human A-RAF around the phosphorylation site of Tyr302.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:20000

Comment:

Unigene-Number: Hs.225936, Hs.446641 (NCBI Gene Symbol: ARAF)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for ARAF

Target: ARAF (V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF))

Alternative Name: A-RAF

Background:

Synonyms: A-Raf proto-oncogene serine/threonine-protein kinase, A-raf-1, Proto-oncogene Pks, ARAF, ARAF1, PKS, PKS2

NCBI Gene Symbol: ARAF

Molecular Weight: 67 kDa

Gene ID: 369

OMIM: 311010

UniProt: P10398

Pathways: MAPK Signaling, RTK Signaling, Hepatitis C