HMGN1 antibody (pSer21)

Catalog No. ABIN482266

This Rabbit Polyclonal antibody specifically detects HMGN1 in IHC, ELISA and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for HMGN1 antibody (pSer21) (ABIN482266)

Target: HMGN1 (High Mobility Group Nucleosome Binding Domain 1 (HMGN1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HMGN1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-HMGN1 Antibody

Binding Specificity: AA 10-59, pSer21

Specificity:

HMG14 (Phospho-Ser21) Antibody detects endogenous levels of HMG14 only when phosphorylated at Ser21.

PhosphorylationH:S21 M:S20 R:S21

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human HMG14 around the phosphorylation site of Ser21.

Isotype: IgG

Application Details

Application Notes: IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000

Comment:

Unigene-Number: Hs.356285, Hs.702472 (NCBI Gene Symbol: HMGN1)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for HMGN1

Target: HMGN1 (High Mobility Group Nucleosome Binding Domain 1 (HMGN1))

Alternative Name: HMG14

Background:

Synonyms: high-mobility group nucleosome binding domain 1, HMG14, nonhistone chromosomal protein HMG-14

NCBI Gene Symbol: HMGN1

Molecular Weight: 10 kDa

Gene ID: 3150

OMIM: 163920

UniProt: P05114

Pathways: Chromatin Binding