KIT antibody (pTyr721)

Catalog No. ABIN482506

This Rabbit Polyclonal antibody specifically detects KIT in WB and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for KIT antibody (pTyr721) (ABIN482506)

Target: KIT (Mast/stem Cell Growth Factor Receptor (KIT))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KIT antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-KIT Antibody

Binding Specificity: AA 688-737, pTyr721

Specificity:

c-Kit (Phospho-Tyr721) Antibody detects endogenous levels of c-Kit only when phosphorylated at Tyr721.

PhosphorylationH:Y721 M:Y719 R:Y722

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human c-Kit around the phosphorylation site of Tyr721.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 ELISA: 1:10000

Comment:

Unigene-Number: Hs.479754 (NCBI Gene Symbol: KIT)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for KIT

Target: KIT (Mast/stem Cell Growth Factor Receptor (KIT))

Alternative Name: C-Kit

Background:

Synonyms: SCFR, Proto-oncogene tyrosine-protein kinase Kit, c-kit, CD117 antigen

NCBI Gene Symbol: KIT

Molecular Weight: 109 kDa

Gene ID: 3815

OMIM: 164920

UniProt: P10721

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Sensory Perception of Sound, Stem Cell Maintenance, Production of Molecular Mediator of Immune Response, Regulation of long-term Neuronal Synaptic Plasticity