MDM2 antibody (AA 132-181)

Catalog No. ABIN482974

This Rabbit Polyclonal antibody specifically detects MDM2 in WB, ELISA and IF. It exhibits reactivity toward Human and Mouse.

Quick Overview for MDM2 antibody (AA 132-181) (ABIN482974)

Target: MDM2 (Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MDM2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Product Details anti-MDM2 Antibody

Binding Specificity: AA 132-181

Specificity:

MDM2 Antibody detects endogenous levels of total MDM2 protein.

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human MDM2.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:1000

Comment:

Unigene-Number: Hs.484551 (NCBI Gene Symbol: MDM2)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for MDM2

Target: MDM2 (Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2))

Alternative Name: MDM2

Background:

Synonyms: Double minute 2 protein, HDM2, Oncoprotein Mdm2, p53-binding protein Mdm2, Ubiquitin-protein ligase E3 Mdm2

NCBI Gene Symbol: MDM2

Molecular Weight: 55 kDa

Gene ID: 4193

OMIM: 164785

UniProt: Q00987

Pathways: p53 Signaling, PI3K-Akt Signaling, Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Autophagy, Ubiquitin Proteasome Pathway