ERCC8 antibody (C-Term)

Catalog No. ABIN486848

The Rabbit Polyclonal anti-ERCC8 antibody has been validated for WB and IHC. It is suitable to detect ERCC8 in samples from Human, Rat, Dog, Rabbit, Horse and Pig. There is 1 publication available.

Quick Overview for ERCC8 antibody (C-Term) (ABIN486848)

Target: ERCC8 (Excision Repair Cross-Complementing Rodent Repair Deficiency, Complementation Group 8 (ERCC8))

Reactivity: Human, Rat, Dog, Rabbit, Horse, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ERCC8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-ERCC8 Antibody

Binding Specificity: C-Term

Sequence: QELYSGSRDC NILAWVPSLY EPVPDDDETT TKSQLNPAFE DAWSSSDEEG

Predicted Reactivity: Dog: 93%, Horse: 86%, Human: 100%, Pig: 79%, Rabbit: 79%, Rat: 79%

Characteristics: This is a rabbit polyclonal antibody against ERCC8. It was validated on Western Blot and immunohistochemistry.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human ERCC8

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 396 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-ERCC8 antibody (ABIN486848)

Fleury, Carmona, Morin, Meunier, Masson, Tonin, Provencher, Mes-Masson: "Cumulative defects in DNA repair pathways drive the PARP inhibitor response in high-grade serous epithelial ovarian cancer cell lines." in: Oncotarget, Vol. 8, Issue 25, pp. 40152-40168, (2018) (PubMed).

Target Details for ERCC8

Target: ERCC8 (Excision Repair Cross-Complementing Rodent Repair Deficiency, Complementation Group 8 (ERCC8))

Alternative Name: ERCC8

Background: ERCC8 (excision repair cross-complementing rodent repair deficiency, complementation group 8 isoform 1) is a WD repeat protein, which interacts with Cockayne syndrome type B (CSB) protein and with p44 protein, a subunit of the RNA polymerase II transcript
Alias Symbols: CKN1, CSA
Protein Interaction Partner: CUL4A, CUL4B, DDB1, RUVBL2, ZNF24, PCNA, H1F0, ERCC6, UVSSA, DDA1, RFWD2, COPS4, COPS6, COPS3, TOP1, TP53, SSBP1, OGG1, MDM2, GTF2H2, CUL5, GPS1, DDB2, COPS2, COPS7A, COPS5, COPS8, RBX1, POLR2A, HMGN1, EP300, UBC, CSNK2B, XAB2, UQCRQ, CBR1,
Protein Size: 396

Molecular Weight: 44 kDa

Gene ID: 1161

NCBI Accession: NM_000082, NP_000073

UniProt: Q13216

Pathways: DNA Damage Repair, Positive Regulation of Response to DNA Damage Stimulus