MAPRE3
Reactivity: Human, Mouse, Rat
WB, IHC (p), FACS, IF (cc), IF (p)
Host: Rabbit
Monoclonal
3C9
unconjugated
Application Notes
Peptide ELISA: 1/64000. Western blot: Approx 32 kDa band observed in Human Brain (Cerebellum and Amygdala)and Mouse Brain lysates (calculated MW of 32 kDa according to NP_036458.2). Recommended concentration: 0.1 - 1.0 μg/mL. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
0.5 mg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
Target
MAPRE3
(Microtubule-Associated Protein, RP/EB Family, Member 3 (MAPRE3))
EB3 antibody, EBF3 antibody, EBF3-S antibody, RP3 antibody, AI790651 antibody, EB2 antibody, MGC68667 antibody, zgc:114065 antibody, fj35g02 antibody, wu:fj35g02 antibody, zgc:91952 antibody, microtubule associated protein RP/EB family member 3 antibody, microtubule-associated protein, RP/EB family, member 3 antibody, microtubule associated protein RP/EB family member 3 S homeolog antibody, microtubule-associated protein, RP/EB family, member 3a antibody, microtubule-associated protein, RP/EB family, member 3b antibody, MAPRE3 antibody, Mapre3 antibody, mapre3.S antibody, mapre3a antibody, mapre3b antibody
Background
EB3 is a 282 amino acid homologue of EB1, a protein known to bind Adenomatous Polyposis Coli. It has been shown to bind to tubulin in vitro and microtubules in vivo. Northern blotting shows expressions to be mainly in brain, and to a lesser degree in muscle. EB3 interacts with APCL, a homologue of the tumor suppressor APC, and this interaction may be involved in the stabilization or extension of microtubules.Synonyms: EB1 protein family member 3, EB3, End-binding protein 3, Microtubule-associated protein RP/EB family member 3, RP3