DIAPH1 antibody (Internal Region)

Catalog No. ABIN488038

Product Details anti-DIAPH1 Antibody

Target: DIAPH1 (Diaphanous Homolog 1 (DIAPH1))

Binding Specificity: Internal Region

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This DIAPH1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: This antibody is expected to recognize both reported isoforms (NP_005210.3 and NP_001073280.1).

Cross-Reactivity (Details): Species reactivity (expected):Human.
Species reactivity (tested):Mouse.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-QTKTSKAKKDQ, from the internal region of the protein sequence according to NP_005210.3 and NP_001073280.1. Genename: DIAPH1

Application Details

Application Notes: Peptide ELISA: Detection Limit Dilution: 1/32000. Western blot: 0.3-1 μg/mL. Approx 140 kDa band observed in lysates of cell line NIH3T3(calculated MW of 140 kDa according to Human NP_005210.3 and 130 kDa according toMouse NP_031884.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for DIAPH1

Target: DIAPH1 (Diaphanous Homolog 1 (DIAPH1))

Alternative Name: DIAPH1 (DIAPH1 Products)

Synonyms: DIAPH1 antibody, dia1 antibody, drf1 antibody, dfna1 antibody, diap1 antibody, lfhl1 antibody, DIAPH2 antibody, Diap1 antibody, D18Wsu154e antibody, Dia1 antibody, Diaph1 antibody, Drf1 antibody, mKIAA4062 antibody, p140mDia antibody, DFNA1 antibody, DIA1 antibody, DRF1 antibody, LFHL1 antibody, hDIA1 antibody, diaphanous related formin 1 antibody, diaphanous related formin 2 antibody, diaphanous-related formin 1 antibody, protein diaphanous homolog 1 antibody, DIAPH1 antibody, diaph1 antibody, DIAPH2 antibody, Diaph1 antibody, LOC100457442 antibody

Background: DIAPH1 binds to GTP-bound form of Rho and to profilin. Acts in a Rho-dependent manner to recruit profilin to the membrane, where it promotes actin polymerization. It is required for cytokinesis, stress fiber formation, and transcriptional activation of the serum response factor. DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics (By similarity). In hearing it may play a role in the regulation of actin polymerization in hair cells.Synonyms: DIAP1, DRF1, Diaphanous homolog 1, Diaphanous-1, Diaphanous-related formin-1

Gene ID: 1729

NCBI Accession: NP_005210

UniProt: O60610

Pathways: Sensory Perception of Sound