EPH Receptor A2 antibody

Catalog No. ABIN488132

Product Details anti-EPH Receptor A2 Antibody

Target: EPH Receptor A2 (EPHA2)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This EPH Receptor A2 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Specificity: Recognizes EphA2 receptor tyrosine kinase (EphA2).

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Affinity Chromatography on Protein G.

Immunogen: Genetic immunisation with cDNA encoding Human EphA2 Remarks: Selection: Based on recognition of the complete native protein expressed on transfected mammalian cells

Clone: GM5H5

Isotype: IgG1

Application Details

Application Notes: cELISA: 1/200-1/400. Flow Cytometry: 1.2 μg/10^6 cells. Immunofluorescence: 1 μg/10^6 cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 2.0 mg/mL

Buffer: Phosphate buffered saline, pH 7.2

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for EPH Receptor A2

Target: EPH Receptor A2 (EPHA2)

Alternative Name: EPHA2 / ECK (EPHA2 Products)

Synonyms: ARCC2 antibody, CTPA antibody, CTPP1 antibody, CTRCT6 antibody, ECK antibody, epha2 antibody, MGC89505 antibody, AW545284 antibody, Eck antibody, Myk2 antibody, Sek-2 antibody, Sek2 antibody, id:ibd5152 antibody, rtk6 antibody, G42 antibody, XE10 antibody, epha2-A antibody, EPH receptor A2 antibody, Eph receptor A2 antibody, epoxide hydrolase antibody, eph receptor A2 a antibody, EPH receptor A2 L homeolog antibody, EPHA2 antibody, Epha2 antibody, epha2 antibody, ephA2 antibody, epha2a antibody, epha2.L antibody

Background: EphA2 (Eph receptor tyrosine kinase A2) belongs to the Eph tyrosine receptor familiy, the largest receptor tyrosine kinase family of transmembrane proteins. It encodes a 130 kDA transmembrane protein which is primarily found in adult human epithelial cells (1). Eph receptors and their ephrin ligands are important mediators of cell-cell communication and play roles in embryonic patterning, neuronal targeting, and vascular development during normal embryogenesis (2,3). The Eph family of receptor tyrosine kinases is frequently overexpressed in a wide variety of cancers and tumor cell lines. In particular, EphA2 is overexpressed in prostate, lung and colon cancers and 40 % of breast cancers and it represent an attractive potential target for drug design (3,4).Synonyms: Eph receptor A2, Ephrin type-A receptor 2, Epithelial cell kinase, Tyrosine-protein kinase receptor ECK

Gene ID: 1969

NCBI Accession: NP_004422

UniProt: P29317

Pathways: RTK Signaling