E-cadherin antibody

Catalog No. ABIN490144

This Mouse Monoclonal antibody specifically detects E-cadherin in WB, IHC, FACS, IP, ICC and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for E-cadherin antibody (ABIN490144)

Target: E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This E-cadherin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 67A4

Product Details anti-E-cadherin Antibody

Specificity: The mouse monoclonal antibody 67A4 recognizes CD324 / E-cadherin, an approximately 100 kD epithelial cell adhesion molecule, whose detection is important for determination of invasive potential of epithelial neoplasms. HLDA VIII.

Purification: Protein A purified

Immunogen: T-47D cells.

Type of Immunogen: Cells

Isotype: IgG1

Application Details

Application Notes: Approved: Flo, ICC, IHC, IHC-Fr, IP, WB

Usage: Flow Cytometry: Application note - Tested on cell lines CACO-2 and HT-29. In this case the recommended concentration is 5-10 μg/mL per 1 million cells/mL. Immunohistochemistry (frozen sections): Recommended dilution - 4-8 μg/mL. Positive tissue - tonsil.

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 15 mM sodium azide,  pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for E-cadherin

Target: E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))

Alternative Name: CDH1 / E Cadherin

Background: Name/Gene ID: CDH1
Family: Cadherin

Synonyms: CDH1, CD324, CDHE, Cell-CAM 120/80, E Cadherin, E-Cadherin, ECAD, Epithelial cadherin, Arc-1, UVO, Cadherin-1, CAM 120/80, CD324 antigen, LCAM, Uvomorulin

Gene ID: 999

UniProt: P12830

Pathways: WNT Signaling, Sensory Perception of Sound, Cell-Cell Junction Organization, Tube Formation