FGF6 antibody
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- Target See all FGF6 Antibodies
- FGF6 (Fibroblast Growth Factor 6 (FGF6))
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Reactivity
- Human
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This FGF6 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Neutralization (Neut), Functional Studies (Func)
- Specificity
- Recognizes human, recombinant Fibroblast Growth Factor 6 (FGF6). ~50 % cross-reactivity with rhFGF4, less than 5 % cross-reactivity with rhFGF9 and less than 2 % cross-reactivity with FGF-acidic, rhFGF5 and rhFGF7. In direct ELISA, this antibody shows no cross-reactivity with other cytokines tested.
- Purification
- Immunoaffinity purified
- Immunogen
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Human, recombinant Fibroblast Growth Factor 6 (FGF6) from E. coli.
Type of Immunogen: Recombinant protein - Isotype
- IgG
- Top Product
- Discover our top product FGF6 Primary Antibody
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- Application Notes
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Approved: ELISA (0.5 - 1 μg/mL), Func, Neut, WB (0.1 - 0.2 μg/mL)
Usage: Suitable for use in Neutralization, Western Blot and ELISA. Neutralization: ND50 is ~0.025-0.075 μg/mL in the presence of 1 ng/mL of rhFGF6 and 1 μg/mL of heparin, using the FGF6 responsive NR6R-3T3 fibroblasts as target cells. The Neutralization Dose50 (ND50) for this antibody is defined as that concentration of antibody required to yield one-half maximal inhibition of the cytokine activity on a responsive cell line, when that cytokine is present at a concentration just high enough to elicit a maximum response. Western Blot: 0.1-0.2 μg/mL with the appropriate secondary reagents to detect human FGF6. The detection limit for rhFGF6 is approximately 2 ng/lane under non-reducing and reducing conditions. ELISA: 0.5-1 μg/mL with the appropriate secondary reagents to detect human FGF6. The detection limit for rhFGF6 is ~0.16 ng/well. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, pH 7.2, 40 % glycerol.
- Handling Advice
- Avoid repeat freeze-thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- Target
- FGF6 (Fibroblast Growth Factor 6 (FGF6))
- Alternative Name
- FGF6 (FGF6 Products)
- Synonyms
- FGF6 antibody, hst2 antibody, fgf-6 antibody, hbgf-6 antibody, HBGF-6 antibody, HST2 antibody, Fgf-6 antibody, HSTF-2 antibody, fgf4 antibody, fgf6 antibody, fibroblast growth factor 6 antibody, fibroblast growth factor 6a antibody, FGF6 antibody, fgf6 antibody, Fgf6 antibody, fgf6a antibody
- Background
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Name/Gene ID: FGF6
Family: HBGF
Synonyms: FGF6, FGF-6, Fibroblast growth factor 6, HSTF-2, HBGF-6, HST-2, HST2, HSTF2 - Gene ID
- 2251
- UniProt
- P10767
- Pathways
- RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
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