N-Cadherin antibody (Cytoplasmic Domain) (Biotin)
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- Target See all N-Cadherin (CDH2) Antibodies
- N-Cadherin (CDH2) (Cadherin 2 (CDH2))
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Binding Specificity
- Cytoplasmic Domain
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Reactivity
- Human, Mouse
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This N-Cadherin antibody is conjugated to Biotin
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Application
- Western Blotting (WB)
- Specificity
- This antibody specifically recognizes N-Cadherin at 127 kDa.
- Characteristics
- Synonyms: CDH2, CDHN, NCAD, Neural cadherin, CDw325, Cadherin N, N-Cadherin
- Purification
- Subsequent Ultrafiltration and Size Exclusion Chromatography.
- Components
- incl. positive Control
- Immunogen
- Peptide conjugated to hemocyanin. Epitope: Cytoplasmic domain
- Clone
- 7-00E-009
- Isotype
- IgG1
- Top Product
- Discover our top product CDH2 Primary Antibody
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- Application Notes
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Immunoblotting: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. Included Positive Control: Cell lysate from untreated SH-SY5Y cells (See Ptotocols for moredetails).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Protocol
- Positive Control: Cell lysate from untreated SH-SY5Y cellsFormat: Lyophilized cell lysate from serum starved SH-SY5Y cells. Reconstitution: Restore by addition of 200 µl H2O. After complete solubilization add 200µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 20. 000 cells. Use 20 µl/lane (mini gel) for HRPO/ECL detection of the target proteins. Please NOTE: The lyophilized cell lysates conatin SDS and are not recommended forapplications with native proteins such as in immunoprecipitation.
- Restrictions
- For Research Use only
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- Concentration
- 0.5 mg/mL
- Buffer
- PBS / 0.09 % Sodium Azide / PEG and Sucrose.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Storage Comment
- Aliquote and freeze in liquid nitrogen. Antibody can be stored frozen at -80 °C up to 1 year. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
- Expiry Date
- 12 months
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- Target
- N-Cadherin (CDH2) (Cadherin 2 (CDH2))
- Alternative Name
- Cadherin-2 (CDH2 Products)
- Synonyms
- CD325 antibody, CDHN antibody, CDw325 antibody, NCAD antibody, N-cadherin antibody, cadherin-2 antibody, cdhn antibody, ncad antibody, cdw325 antibody, CDH2 antibody, Cadherin-2 antibody, Ncad antibody, glo antibody, lyr antibody, pac antibody, wu:fb47h04 antibody, cadherin 2 antibody, cadherin 2 S homeolog antibody, cadherin 2, type 1, N-cadherin (neuronal) antibody, CDH2 antibody, cdh2.S antibody, cdh2 antibody, Cdh2 antibody
- Background
- Cadherins are a family of transmembrane glycoproteins that play a key role in calcium-dependent cell-cell adhesion. Several members of the cadherin family have been identified so far, including E- (epithelial), P- (placental), N- (neuronal), and M- (muscle) cadherin.Synonyms: CDH2, CDHN, CDw325, Cadherin N, N-Cadherin, NCAD, Neural cadherin
- Gene ID
- 1000
- UniProt
- P19022
- Pathways
- Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Synaptic Membrane
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