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GAPDH antibody (Glyceraldehyde-3-Phosphate Dehydrogenase) (AA 1-335)

Details for Product anti-GAPDH Antibody No. ABIN492380, Supplier: Log in to see
Antigen
  • BEST:GH12586
  • cb609
  • CG8893
  • CG12055
  • Dmel\\CG8893
  • Dmel\\CG12055
  • g3pd
  • G3PD
  • G3PDH
  • GA3PDH
  • GADPH
  • gadph
  • GAP
  • GAPC-2
  • gapd
  • GAPD
  • Gapd
  • gapdh
  • GAPDH
  • Gapdh
  • GAPDH-1
  • Gapdh-1
  • gapdh-1
  • Gapdh-2
  • GAPDH1
  • GAPDH2
  • Gapdh13F
  • Gapdh43E
  • GAPDHI
  • GAPDH I
  • GAPDH II
  • GB14798
  • gh12586
  • GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE C-2
  • glyceraldehyde-3-phosphate dehydrogenase C2
  • KNC-NDS6
  • mg:bb02e05
  • T6J4.17
  • T6J4_17
  • wu:fb33a10
  • wu:ft80f05
Epitope
AA 1-335, N-Term
113
45
29
27
17
10
9
9
8
8
7
7
6
6
5
5
5
5
4
4
4
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
672
326
271
141
105
62
60
52
44
42
34
32
31
25
22
17
16
15
15
15
13
13
10
8
8
7
6
6
6
6
6
4
4
4
3
3
2
1
1
1
1
1
1
1
1
1
1
Host
Mouse
369
327
58
32
20
Clonality (Clone)
Monoclonal ()
Conjugate
This GAPDH antibody is un-conjugated
50
41
21
14
12
12
10
10
10
9
9
8
6
6
6
6
5
4
4
3
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
724
341
245
168
160
128
46
30
25
24
16
16
15
10
7
6
5
5
5
4
4
2
2
1
1
1
1
Supplier
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Immunogen Recombinant human GAPDH (1-335aa) purified from E. coli
Clone AT8G4
Isotype IgG2b
Specificity The antibody recognizes GAPDH.
Cross-Reactivity (Details) Species reactivity (tested):Human.
Characteristics Synonyms: GAPD, CDABP0047, Loading control, Glyceraldehyde-3-Phosphate Dehydrogenase
Purification Protein-G affinity chromatography
Alternative Name GAPDH (GAPDH Antibody Abstract)
Background Multiple roles for glyceraldehyde-3-phosphate dehydrogenase (GAPDH) have been recently appreciated. GAPDH is found in the particulate fractions, such as the nucleus, the mitochondria, and the small vesicular fractions. GAPDH gene expression is specifically increased during programmed neuronal cell death. When cells are exposed to various stressors, dynamic subcellular re-distribution of GAPDH occurs. GAPDH is also involved in various diseases, especially neurodegenerative disorders and cancers. As a membrane protein, GAPDH functions in endocytosis, in the cytoplasm, it is involved in the translational control of gene expression, in the nucleus, it functions in nuclear tRNA export, in DNA replication, and in DNA repair.Synonyms: CDABP0047, GAPD, Glyceraldehyde-3-Phosphate Dehydrogenase
Gene ID 2597
UniProt P04406
Research Area Cancer, Metabolism, Alzheimer's Disease, Enzymes
Application Notes ELISA. Western blot (1: 500 - 1: 10,000). Immunofluorescence (1: 500 - 1: 10,000).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4, 0.09 % Sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months
Supplier Images
Western Blotting (WB) image for anti-GAPDH antibody (Glyceraldehyde-3-Phosphate Dehydrogenase) (AA 1-335) (ABIN492380) anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 1-335), (N-Term) antibody
Immunofluorescence (IF) image for anti-GAPDH antibody (Glyceraldehyde-3-Phosphate Dehydrogenase) (AA 1-335) (ABIN492380) anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 1-335), (N-Term) antibody (Image 2)
Western Blotting (WB) image for anti-GAPDH antibody (Glyceraldehyde-3-Phosphate Dehydrogenase) (AA 1-335) (ABIN492380) anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 1-335), (N-Term) antibody (Image 3)
Immunofluorescence (IF) image for anti-GAPDH antibody (Glyceraldehyde-3-Phosphate Dehydrogenase) (AA 1-335) (ABIN492380) anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 1-335), (N-Term) antibody (Image 4)
Background publications Chuang, Hough, Senatorov: "Glyceraldehyde-3-phosphate dehydrogenase, apoptosis, and neurodegenerative diseases." in: Annual review of pharmacology and toxicology, Vol. 45, pp. 269-90, 2005 (PubMed).

Mazzola, Sirover: "Alteration of intracellular structure and function of glyceraldehyde-3-phosphate dehydrogenase: a common phenotype of neurodegenerative disorders?" in: Neurotoxicology, Vol. 23, Issue 4-5, pp. 603-9, 2002 (PubMed).