KCNT1 antibody (AA 1168-1237)

Catalog No. ABIN492453

Product Details anti-KCNT1 Antibody

Target: KCNT1 (Potassium Channel, Subfamily T, Member 1 (KCNT1))

Binding Specificity: AA 1168-1237

Reactivity: Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This KCNT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: Detects ~140 kDa protein.

Cross-Reactivity (Details): Species reactivity (expected):Human (weak), Mouse.
Species reactivity (tested):Rat.

Characteristics: Synonyms: KIAA1422, KCa4.1, Potassium channel subfamily T member 1

Purification: Protein G Chromatography

Immunogen: Fusion protein amino acids 1168-1237 of Rat Slo2. 2 (Slack, NP_068625)

Clone: S3-26

Isotype: IgG1

Application Details

Application Notes: Western blot: 1 μg/mL (If results are poor use lysate without boiling, heat at 37 °C for 15 minutes).1 μg/mL was sufficient for detection of Slo2.2 in 10 μg of rat brain lysate by colorimetricimmunoblot analysis using Goat anti-mouse IgG: HRP as the secondary antibody. Immunoprecipitation: 1.0-10 μg/mLImmunofluorescence: 1.0-10 μg/mLImmunocytochemistry: 0.1-1.0 μg/mLImmunohistochemistry: 0.1-1.0 μg/mL
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 50 % Glycerol as stabilizer and 0.09 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for KCNT1

Target: KCNT1 (Potassium Channel, Subfamily T, Member 1 (KCNT1))

Alternative Name: KCNT1 (KCNT1 Products)

Synonyms: EIEE14 antibody, ENFL5 antibody, KCa4.1 antibody, SLACK antibody, bA100C15.2 antibody, Slack antibody, rSlo2 antibody, C030030G16Rik antibody, mKIAA1422 antibody, slo2 antibody, slack antibody, Slo2.2 antibody, MGC146594 antibody, potassium sodium-activated channel subfamily T member 1 antibody, potassium channel subfamily T member 1 antibody, potassium channel, subfamily T, member 1 antibody, potassium channel, sodium activated subfamily T, member 1 antibody, KCNT1 antibody, LOC100436526 antibody, Kcnt1 antibody, kcnt1 antibody

Background: Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient (1). They are present in the membranes that surround all biological cells because their main function is to regulate the flow of ions across this membrane. Whereas some ion channels permit the passage of ions based on charge, others conduct based on a ionic species, such as sodium or potassium. Furthermore, in some ion channels, the passage is governed by a gate which is controlled by chemical or electrical signals, temperature, or mechanical forces. There are a few main classifications of gated ion channels. There are voltage- gated ion channels, ligandgated, other gating systems and finally those that are classified differently, having more exotic characteristics. The first are voltage- gated ion channels which open and close in response to membrane potential. These are then separated into sodium, calcium, potassium, proton, transient receptor, and cyclic nucleotide-gated channels, each of which is responsible for a unique role. Ligand-gated ion channels are also known as ionotropic receptors, and they open in response to specific ligand molecules binding to the extracellular domain of the receptor protein. The other gated classifications include activation and inactivation by second messengers, inwardrectifier potassium channels, calcium-activated potassium channels, two-pore-domain potassium channels, light-gated channels, mechano-sensitive ion channels and cyclic nucleotide-gated channels. Finally, the other classifications are based on less normal characteristics such as two-pore channels, and transient receptor potential channels (2). Slo2.2 is a novel member of the mammalian Slo potassium channel gene family. Slo2 channels may contribute to the resting potentials of cells that control their basal level of excitability (3). They also have sensors that couple channel activity to the intracellular concentrations of Na+ and Cl-(4).Synonyms: KCa4.1, KIAA1422, Potassium channel subfamily T member 1

Gene ID: 60444

UniProt: Q9Z258