KCNU1 antibody (AA 1052-1121)

Catalog No. ABIN492456

Product Details anti-KCNU1 Antibody

Target: KCNU1 (Potassium Channel, Subfamily U, Member 1 (KCNU1))

Binding Specificity: AA 1052-1121

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This KCNU1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunoprecipitation (IP)

Specificity: Detects ~115 kDa protein.

Characteristics: Synonyms: KCNU1, KCNMA3, KCNMC1, KCa5, Slowpoke homolog 3, Potassium channel subfamily Umember 1, Calcium-activated potassium channel subunit alpha-3

Purification: Protein G Chromatography.

Immunogen: Fusion protein amino acids 1052-1121 of Mouse Slo3 (accession number AAB99742).

Clone: S2-16

Isotype: IgG2b

Application Details

Application Notes: Western blot: 1 μg/mL1 μg/mL was sufficient for detection of Slo3 in 10 μg of rat brain lysate by colorimetricimmunoblot analysis using Goat anti-mouse IgG: HRP as the secondary antibody. Immunoprecipitation: 1.0-10 μg/mLImmunofluorescence: 1.0-10 μg/mLImmunocytochemistry: 0.1-1.0 μg/mLImmunohistochemistry: 0.1-1.0 μg/mL
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 50 % Glycerol as stabilizer and 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for KCNU1

Target: KCNU1 (Potassium Channel, Subfamily U, Member 1 (KCNU1))

Alternative Name: KCNU1 / SLO3 (KCNU1 Products)

Synonyms: Kcnma3 antibody, Slo3 antibody, mSlo3 antibody, KCNMC1 antibody, KCa5 antibody, KCa5.1 antibody, potassium channel, subfamily U, member 1 antibody, potassium calcium-activated channel subfamily U member 1 antibody, Kcnu1 antibody, KCNU1 antibody

Background: Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient (1). They are present in the membranes that surround all biological cells because their main function is to regulate the flow of ions across this membrane. Whereas some ion channels permit the passage of ions based on charge, others conduct based on a ionic species, such as sodium or potassium. Furthermore, in some ion channels, the passage is governed by a gate which is controlled by chemical or electrical signals, temperature, or mechanical forces. There are a few main classifications of gated ion channels. There are voltage- gated ion channels, ligandgated, other gating systems and finally those that are classified differently, having more exotic characteristics. The first are voltage- gated ion channels which open and close in response to membranepotential. These are then separated into sodium, calcium, potassium, proton, transient receptor, and cyclic nucleotide-gated channels, each of which is responsible for a unique role. Ligand-gated ion channels are also known as ionotropic receptors, and they open in response to specific ligand molecules binding to the extracellular domain of the receptor protein. The other gated classifications include activation and inactivation by second messengers, inward-rectifier potassium channels, calcium-activated potassium channels, two-pore-domain potassium channels, light-gated channels, mechano-sensitive ion channels and cyclic nucleotide-gated channels. Finally, the other classifications are based on less normal characteristics such as two-pore channels, and transient receptor potential channels (2). The Slo3 channel is a novel potassium channel abundantly expressed in mammalian speramtocytes- tests have shown that it is expressed in both mouse and human testis (3). It represents a new and unique type of potassium channel that is regulated by both intracellular pH and membrane voltage (3). Because of its sensitivity to both pH and voltage, Slo3 may play a role in alkalization-mediated K(+) fluxes associated with sperm capacitation (4).Synonyms: Calcium-activated potassium channel subunit alpha-3, KCNMA3, KCNMC1, KCa5, Potassium channel subfamily U member 1, Slowpoke homolog 3, pH -sensitive maxi potassium channel

Gene ID: 16532

UniProt: O54982