KCNU1 antibody

Catalog No. ABIN6657909

Product Details anti-KCNU1 Antibody

Target: KCNU1 (Potassium Channel, Subfamily U, Member 1 (KCNU1))

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This KCNU1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Fluorescence Microscopy (FM)

Purification: Anti-mSlo3 Antibody was purified by Protein G chromatography. This monoclonal antibody is specific for mouse Slo3 protein. A BLAST analysis was used to suggest cross-reactivity with mSlo3 from mouse based on 100% homology with the immunizing sequence. Cross-reactivity with mSlo3 from other sources has not been determined. Ion Channels research.

Immunogen:

Immunogen: mSlo3 Antibody was produced in mice by repeated immunizations raised against a fusion protein of mouse Slo3.

Immunogen Type: Recombinant Protein

Clone: S2-16

Isotype: IgG2b

Application Details

Application Notes:

Immunohistochemistry Dilution: 0.1-1.0 μg/mL

Application Note: Anti-Slo3 Antibody is suitable for use in WB and IF microscopy (mouse sperm). Expect a band approximately ~115 kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: 1-10 μg/mL

IF Microscopy Dilution: 1.0-10 μg/mL

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Storage: RT,4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Target Details for KCNU1

Target: KCNU1 (Potassium Channel, Subfamily U, Member 1 (KCNU1))

Alternative Name: mSlo3 (KCNU1 Products)

Synonyms: Kcnma3 antibody, Slo3 antibody, mSlo3 antibody, KCNMC1 antibody, KCa5 antibody, KCa5.1 antibody, potassium channel, subfamily U, member 1 antibody, potassium calcium-activated channel subfamily U member 1 antibody, Kcnu1 antibody, KCNU1 antibody

Background:

Synonyms: mouse anti-mSlo3 antibody, mouse anti-Slo3 antibody, Potassium channel subfamily U member 1, Calcium-activated potassium channel subunit alpha-3, Calcium-activated potassium channel, subfamily M subunit alpha-3, Slowpoke homolog 3, mSlo3, pH -sensitive maxi potassium channel, Kcnu1, Kcnma3, Slo3

Background: The Slo3 channel is a novel potassium channel abundantly expressed in mammalian speramtocytes- tests have shown that it is expressed in both mouse and human testis. It represents a new and unique type of potassium channel that is regulated by both intracellular pH and membrane voltage. Because of its sensitivity to both pH and voltage, Slo3 may play a role in alkalization-mediated K(+) fluxes associated with sperm capacitation.

Gene Name: Kcnu1

Gene ID: 16532

NCBI Accession: NP_032458

UniProt: O54982