KCNAB2 antibody (N-Term)

Catalog No. ABIN492482

Product Details anti-KCNAB2 Antibody

Target: KCNAB2 (Potassium Voltage-Gated Channel, Shaker-Related Subfamily, beta Member 2 (KCNAB2))

Binding Specificity: AA 1-367, N-Term

Reactivity: Rat, Human, Mouse, Zebrafish (Danio rerio)

Host: Mouse

Clonality: Monoclonal

Conjugate: This KCNAB2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)

Specificity: Detects a major band at ~38 kDa and a minor band at ~41 kDa.

Characteristics: Synonyms: Voltage-gated potassium channel subunit beta-2, Kv-beta-2, HKvbeta2, KCNA2B, KCNK2

Purification: Protein G Chromatography.

Immunogen: Fusion protein amino acids 1-367 (full length) of rat Kvbeta-2 (accession numberNP_034728).

Clone: S17-70

Isotype: IgG1

Application Details

Application Notes: Western blot: 1 μg/mL1 μg/mL was sufficient for detection of Kvbeta2 in 10 μg of Rat brain lysate by colorimetricimmunoblot analysis using Goat anti-mouse IgG: HRP as the secondary antibody. Immunoprecipitation: 1.0-10 μg/mLImmunofluorescence: 1.0-10 μg/mLImmunocytochemistry: 0.1-1.0 μg/mLImmunohistochemistry: 0.1-1.0 μg/mL
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 50 % Glycerol as stabilizer and 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for KCNAB2

Target: KCNAB2 (Potassium Voltage-Gated Channel, Shaker-Related Subfamily, beta Member 2 (KCNAB2))

Alternative Name: KCNAB2 (KCNAB2 Products)

Synonyms: kcnab2 antibody, KCNAB2 antibody, DKFZp459E056 antibody, akr6a5 antibody, kcna2b antibody, kvb-a antibody, kvb2 antibody, kvbeta2 antibody, kvbeta2.1 antibody, kvbeta2.2 antibody, AKR6A5 antibody, HKvbeta2 antibody, HKvbeta2.1 antibody, HKvbeta2.2 antibody, KCNA2B antibody, KV-BETA-2 antibody, Kvbeta2.1 antibody, F5 antibody, I2rf5 antibody, Kcnb3 antibody, kv-beta-2 antibody, potassium channel, voltage gated subfamily A regulatory beta subunit 1 antibody, potassium voltage-gated channel subfamily A regulatory beta subunit 2 antibody, potassium voltage-gated channel, shaker-related subfamily, beta member 2 a antibody, potassium channel, voltage gated subfamily A regulatory beta subunit 2 L homeolog antibody, voltage-gated potassium channel subunit beta-2 antibody, potassium voltage-gated channel, shaker-related subfamily, beta member 2 antibody, kcnab1 antibody, KCNAB2 antibody, kcnab2a antibody, kcnab2.L antibody, LOC397248 antibody, Kcnab2 antibody

Background: Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient (1). They are present in the membranes that surround all biological cells because their main function is to regulate the flow of ions across this membrane. Whereas some ion channels permit the passage of ions based on charge, others conduct based on a ionic species, such as sodium or potassium. Furthermore, in some ion channels, the passage is governed by a gate which is controlled by chemical or electrical signals, temperature, or mechanical forces. There are a few main classifications of gated ion channels. There are voltage- gated ion channels, ligandgated, other gating systems and finally those that are classified differently, having more exotic characteristics. The first are voltage- gated ion channels which open and close in response to membrane potential. These are then separated into sodium, calcium, potassium, proton, transient receptor, and cyclic nucleotide-gated channels, each of which is responsible for a unique role. Ligand-gated ion channels are also known as ionotropic receptors, and they open in response to specific ligand molecules binding to the extracellular domain of the receptor protein. The other gated classifications include activation and inactivation by second messengers, inwardrectifier potassium channels, calcium-activated potassium channels, two-pore-domain potassium channels, light-gated channels, mechano-sensitive ion channels and cyclic nucleotide-gated channels. Finally, the other classifications are based on less normal characteristics such as two-pore channels, and transient receptor potential channels (2). Depolarization-activated postassium channels (Kv) play a major role in shaping the electrical signals in the nervous system (3). KvBeta2, otherwise known as potassium voltagegated channel, shaker-related subfamily beta member 2, is encoded by the KCNAB2 gene in humans. The KvBeta channels are also members of the aldo-keto reductase (AKR) subfamily. These proteins regulated inactivation and membrane localization of Kv1 and Kv4 channels (4, 5). Specifically Kv2beta2 contributes to regulation of excitability in vivo, although not directly through either chaperone-like or typical AKR catalytic activity (5).Synonyms: HKvbeta2, KCNA2B, KCNK2, Kv-beta-2, Voltage-gated potassium channel subunit beta-2

Gene ID: 16498

UniProt: P62482