TDGF1 antibody (Internal Region)

Catalog No. ABIN498045

The Goat Polyclonal anti-TDGF1 antibody (ABIN498045) specifically detects TDGF1 in IHC (p) and EIA. The antibody is reactive with Human, Mouse, Rat, Cow and Dog samples.

Quick Overview for TDGF1 antibody (Internal Region) (ABIN498045)

Target: TDGF1 (Teratocarcinoma-Derived Growth Factor 1 (TDGF1))

Reactivity: Human, Mouse, Rat, Cow, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This TDGF1 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-TDGF1 Antibody

Binding Specificity: Internal Region

Sequence: YGRNCEHDVR KEN

Specificity: Recognizes CRIPTO

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Canine (Dog), Bovine.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_003203.1. Genename: TDGF1

Application Details

Application Notes: Peptide ELISA: Detection Limit: 1/128000. Western blot: Preliminary experiments in Human Umbilical Cord lysates and in lysates ofcell lines HEK293 and NIH3T3 gave no specific signal but low background (at antibodyconcentration up to 2 μg/mL). Immunohistochemistry: 3-5 μg/mLIn paraffin embedded Human Placenta shows stainng assocated with nuclei in sometrophoblasts and weak cytoplasm staining in cytotrophoblasts.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20 °C.

Target Details for TDGF1

Target: TDGF1 (Teratocarcinoma-Derived Growth Factor 1 (TDGF1))

Alternative Name: TDGF1 / CRIPTO

Background: Human Cripto-1 (CR-1), also known as Teratocarcinoma-derived growth factor 1 (TDGF1), is a member of the epidermal growth factor (EGF)-GFC family and has been implicated in both embryogenesis and carcinogenesis. During early vertebrate development, CR-1 functions as a co-receptor for Nodal, a transforming growth factor beta (TGFbeta) family member, and is essential for mesoderm and endoderm formation and anterior-posterior and left-right axis establishment. In adult tissues, CR-1 is expressed at a low level in all stages of mammary gland development, and expression increases during pregnancy and lactation. Over-expression of CR-1 in mouse mammary epithelial cells leads to their transformation in vitro, and when injected in mammary glands, CR-1 produces ductal hyperplasias.Synonyms: CRGF, Cripto-1 growth factor, Epidermal growth factor-like cripto protein CR1, Teratocarcinoma-derived growth factor 1

Gene ID: 6997

NCBI Accession: NP_003203

UniProt: P13385

Pathways: EGFR Signaling Pathway