Verified
ACAT1
Reactivity: Human, Mouse, Rat
WB, ELISA, IF
Host: Goat
Polyclonal
unconjugated
Application Notes
Peptide ELISA: Detection Limit: 1/8000. Western blot: 0.01-0.03 μg/mL. Detects a band of Approx 45 kDa in Human Liver lysates (calculated MW of 45.5 kDaaccording to NP_000010.1). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
ACAT1, acetoacetyl CoA thiolase, plays a major role in ketone body metabolism. It is a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in the gene encoding ACAT1 are associated with the alpha-methylacetoaceticaciduria disorder,an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone.Synonyms: Acetoacetyl-CoA thiolase T2, Acetyl-CoA acetyltransferase mitochondrial