This antibody is expected to recognize all reported isofoms (NP_001123645.1, NP_005366.2, NP_001123644.1, NP_001155128.1, NP_001155129.1, NP_001155130.1, NP_001155131.1, NP_001155132.1).
Cross-Reactivity (Details)
Species reactivity (expected):Mouse, Rat, Canine (Dog), Bovine, Pig. Species reactivity (tested):Human.
Purification
Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.
Immunogen
Peptide corresponding to internal region of Human c-Myb Genename: MYB
MYB
Reactivity: Human
WB, IHC, ICC
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Peptide ELISA: Detection Limit: 1/8000. Western blot: 0.5-1.5 μg/mL. Detects a band of Approx 85 kDa in lysates of cell line Jurkat. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
0.5 mg/mL
Buffer
Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Myb is a transcriptional activator, DNA-binding protein that recognizes the sequence 5'-YAAC[GT]G-3'. It participates in the control of proliferation and differentiation of hematopoietic progenitor cells. Myb is a transcription factor related to Myc, and is expressed predominantly in haematopoietic cells and immature gastroepithelial cells. TRAF7 stimulates the sumoylation of Myb at Lys-523 and Lys-499, which are the same sites as those used for PIASy-induced sumoylation. A correlation has been established between reduction in sumoylation of Myb and increase in transcriptional activation. Negative influence on transactivation properties by the negative regulatory domain region of c-Myb depends on upon sumoylation.Synonyms: Proto-oncogene c-Myb, Transcriptional activator Myb, c-Myb