NDUFS1
Reactivity: Human, Rat
WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro)
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Peptide ELISA: Detection Limit: 1/64000. Western blot: 0.3-1 μg/mL. Detects a band of Approx 80 kDa in Human Heart and Mouse Heart lysates. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. Species Reactivity: Tested: Human, Mouse. Expected from sequence similarity: Rat, Canine (Dog), Bovine and RabbitAdd. Information: Calculated Molecular Weight: 79.0 kDa according to NP_004997.4
Restrictions
For Research Use only
Concentration
0.5 mg/mL
Buffer
Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
NDUFS1 belongs to the complex I 75 kDa subunit family. Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. Ndufs1 has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. This protein is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized. Mutations in Ndufs1 gene are associated with complex I deficiency.Synonyms: CI-75kD, Complex I-75kD, NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial