MKL1 antibody

Catalog No. ABIN498351

Product Details anti-MKL1 Antibody

Target: MKL1 (Megakaryoblastic Leukemia (Translocation) 1 (MKL1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MKL1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects endogenous levels of MRTF-A protein. (region surrounding His11)

Cross-Reactivity (Details): Species reactivity (tested):Human and Mouse.

Purification: Affinity Chromatography using epitope-specific immunogen.

Application Details

Application Notes: Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for MKL1

Target: MKL1 (Megakaryoblastic Leukemia (Translocation) 1 (MKL1))

Abstract: MKL1 Products

Synonyms: MKL1 antibody, BSAC antibody, MAL antibody, MRTF-A antibody, Mrtf-a antibody, mrtf-a antibody, mrtfa antibody, AI852829 antibody, AMKL antibody, AW743281 antibody, AW821984 antibody, Bsac antibody, Mal antibody, Mrtf-A antibody, megakaryoblastic leukemia (translocation) 1 antibody, megakaryoblastic leukemia 1 protein antibody, megakaryoblastic leukemia (translocation) 1 S homeolog antibody, MKL (megakaryoblastic leukemia)/myocardin-like 1 antibody, MKL1 antibody, mkl1 antibody, Mkl1 antibody, mkl1.S antibody

Background: Serum response factor (SRF) is a transcription factor that binds the serum response element (SRE), a sequence that mediates the transient response of many cellular genes to growth stimulation. SRF regulates the transient response of several muscle genes in response to growth factors and recruits accessory myogenic factors to activate these muscle genes. SRF is required for the formation of vertebrate mesoderm leading to the origin of the cardiovascular system. Myocardin, in association with SRF in cardiac muscle cells, activates cardiac muscle promoters. Myocardin-related transcription factor A (MRTF-A), also known as MKL1, interacts with SRF and stimulates its transcriptional activity.Synonyms: Basic SAP coiled-coil transcription activator, Bsac, MKL, Megakaryoblastic leukemia 1 protein homolog, Mkl1, Myocardin-related transcription factor A

Molecular Weight: approx. 99 kDa

Gene ID: 57591

NCBI Accession: NP_065882

UniProt: Q969V6