AIF antibody (C-Term)

Catalog No. ABIN499239

The Rabbit Polyclonal anti-AIF antibody (ABIN499239) specifically detects AIF in WB, IF and EIA. The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for AIF antibody (C-Term) (ABIN499239)

Target: AIF (AIFM1) (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AIF antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Product Details anti-AIF Antibody

Binding Specificity: AA 593-606, C-Term

Specificity: This antibody detects AIF at C-term.

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse, Rat

Purification: Affinity chromatography purified via peptide column

Immunogen: Peptide corresponding to amino acids 593 to 606 of Human AIF .Remarks: This sequence is identical to those of Mouse and Rat AIF.

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot: 0.25 to 1 μg/mL. A 67 kDa band should be detected. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for AIF

Target: AIF (AIFM1) (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1))

Alternative Name: AIFM1 / AIF

Background: Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins (1). A novel gene, the product of which causes chromatin condensation and DNA fragmentation, was recently identified, cloned, and designated apoptosis inducing factor (AIF) (2). Like the critical molecules, cytochrome c and caspase-9, in apoptosis, AIF localizes in mitochondria. AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. AIF induces chromatin condensation and DNA fragmentation, which are the hallmarks of apoptosis, of the isolated nucleus and the nucleus in live cells by microinjection. AIF is highly conserved between human and mouse and widely expressed (2).Synonyms: Apoptosis-inducing factor 1 mitochondrial, PDCD8, Programmed cell death protein 8

Gene ID: 9131

NCBI Accession: NP_004199

UniProt: O95831

Pathways: Apoptosis, Positive Regulation of Endopeptidase Activity, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Warburg Effect