Autophagy/beclin-1 Regulator 1 (AMBRA1) (N-Term) antibody

Details for Product No. ABIN499273
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Antigen
Synonyms 2310079H06Rik, A130023A14, AA474864, AV021921, D030051N19Rik, mKIAA1736, Nyw1, DCAF3, WDR94
Epitope
N-Term
(19), (11), (2), (2), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine)
(52), (45), (30), (13), (12), (1)
Host
Rabbit
(59), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(51), (28), (27), (14), (10), (7), (1), (1), (1)
Pubmed 4 references available
Quantity 0.1 mg
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Catalog No. ABIN499273
401.50 $
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Immunogen Ambra1 antibody was raised against a 18 amino acid peptide from near the amino terminus of human Ambra1.
Isotype IgG
Specificity This antibody reacts to AMBRA1.
Purification Affinity chromatography purified via peptide column
Alternative Name AMBRA1
Background Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. Beclin-1, a principal regulator of autophagosome formation, is in turn regulated by Ambra1. Ambra1 associates with Beclin-1 through a region near its center as determined by yeast two-hybrid assay. Null mutations in this gene in mice resulted in embryonic lethality with severe neural tube defects associated with autophagy impairment, accumulation of ubiquitinated proteins, unbalanced cell proliferation and excessive apoptotic death . Furthermore, down- regulation of Ambra1 in cultured cells though RNA interference decreased the level of rapamycin- and nutrient starvation-induced autophagy. Multiple isoforms of Ambra1 are known to exist.
Alternate names: Activating molecule in BECN1-regulated autophagy protein 1, KIAA1736
Gene ID 55626
UniProt Q9C0C7
Research Area Cancer, Autophagy, Neurogenesis
Application Notes ELISA. Western Blot: Ambra1 antibody can be used for the detection of Ambra1 at 2 – 4 μg/ml. Immunohistochemistry. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Buffer PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8°C.
Expiry Date 12 months
Background publications Kisen, Tessitore, Costelli et al.: "Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells." in: Carcinogenesis, Vol. 14, Issue 12, pp. 2501-5, 1994 (PubMed).

Liang, Jackson, Seaman et al.: "Induction of autophagy and inhibition of tumorigenesis by beclin 1." in: Nature, Vol. 402, Issue 6762, pp. 672-6, 2000 (PubMed).

Gozuacik, Kimchi: "Autophagy as a cell death and tumor suppressor mechanism." in: Oncogene, Vol. 23, Issue 16, pp. 2891-906, 2004 (PubMed).

Fimia, Stoykova, Romagnoli et al.: "Ambra1 regulates autophagy and development of the nervous system." in: Nature, Vol. 447, Issue 7148, pp. 1121-5, 2007 (PubMed).

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