Autophagy/beclin-1 Regulator 1 (AMBRA1) (N-Term) antibody

Details for Product No. ABIN499273
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Synonyms 2310079H06Rik, A130023A14, AA474864, AV021921, D030051N19Rik, mKIAA1736, Nyw1, DCAF3, WDR94
(26), (19), (12), (4), (1), (1), (1), (1), (1), (1)
Human, Mouse (Murine)
(76), (50), (34), (1), (1)
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(66), (40), (32), (18), (10), (8), (6), (4), (3), (1), (1)
Pubmed 4 references available
Quantity 0.1 mg
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Availability Will be delivered in 6 to 8 Business Days
Immunogen Ambra1 antibody was raised against a 18 amino acid peptide from near the amino terminus of human Ambra1.
Isotype IgG
Specificity This antibody reacts to AMBRA1.
Purification Affinity chromatography purified via peptide column
Alternative Name AMBRA1 (AMBRA1 Antibody Abstract)
Background Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. Beclin-1, a principal regulator of autophagosome formation, is in turn regulated by Ambra1. Ambra1 associates with Beclin-1 through a region near its center as determined by yeast two-hybrid assay. Null mutations in this gene in mice resulted in embryonic lethality with severe neural tube defects associated with autophagy impairment, accumulation of ubiquitinated proteins, unbalanced cell proliferation and excessive apoptotic death . Furthermore, down-regulation of Ambra1 in cultured cells though RNA interference decreased the level of rapamycin- and nutrient starvation-induced autophagy. Multiple isoforms of Ambra1 are known to exist.Synonyms: Activating molecule in BECN1-regulated autophagy protein 1, KIAA1736
Gene ID 55626
UniProt Q9C0C7
Research Area Cancer, Autophagy, Neurogenesis
Application Notes ELISA. Western Blot: Ambra1 antibody can be used for the detection of Ambra1 at 2 - 4 μg/mL. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Buffer PBS containing 0.02 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C.
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Autophagy/beclin-1 Regulator 1 (AMBRA1) (N-Term) antibody (ABIN499273) Immunohistochemistry of Ambra1 in human brain with AP30048PU-N Ambra1 antibody at 5 μ...
Western Blotting (WB) image for anti-Autophagy/beclin-1 Regulator 1 (AMBRA1) (N-Term) antibody (ABIN499273) Western blot analysis of Ambra1 in rat brain tissue lysate with AP30048PU-N Ambra1 an...
Background publications Fimia, Stoykova, Romagnoli et al.: "Ambra1 regulates autophagy and development of the nervous system." in: Nature, Vol. 447, Issue 7148, pp. 1121-5, 2007 (PubMed).

Gozuacik, Kimchi: "Autophagy as a cell death and tumor suppressor mechanism." in: Oncogene, Vol. 23, Issue 16, pp. 2891-906, 2004 (PubMed).

Liang, Jackson, Seaman et al.: "Induction of autophagy and inhibition of tumorigenesis by beclin 1." in: Nature, Vol. 402, Issue 6762, pp. 672-6, 2000 (PubMed).

Kisen, Tessitore, Costelli et al.: "Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells." in: Carcinogenesis, Vol. 14, Issue 12, pp. 2501-5, 1994 (PubMed).

Catalog No. ABIN499273
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