APC2 (Center) antibody

Details for Product No. ABIN499293
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Antigen
Synonyms APCL, AI852447, R75424
Epitope
Center
(6), (3), (3), (1), (1)
Reactivity
Human, Mouse (Murine)
(32), (22), (14), (14), (12)
Host
Rabbit
(32)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
ELISA, Western Blotting (WB)
(23), (11), (10), (9), (9), (3), (2), (1)
Pubmed 4 references available
Quantity 0.1 mg
Shipping to United States (Change)
Availability Will be delivered in 6 to 8 Business Days
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Catalog No. ABIN499293
401.50 $
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Immunogen APC2 antibody was raised against an 18 amino acid peptide near the center of human APC2.
Isotype IgG
Purification Affinity chromatography purified via peptide column
Background Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis. APC2 is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle- associated proteins ensuring that events of mitosis take place in proper sequence. The individual APC/C components mRNA and protein levels are expressed at approximately the same levels in most tissues and cell lines, suggesting that they perform their functions as part of a complex. Like APC11, APC2 contains cullin and RING finger domains that are thought to be important in regulating ubiquitination activity.
Alternate names: APC-like, APCL, Adenomatous polyposis coli protein 2, Adenomatous polyposis coli protein- like
Gene ID 10297
UniProt O95996
Research Area Chromatin and Nuclear Signaling, Transcription Factors, Coagulation
Application Notes ELISA. Western Blot: APC2 antibody can be used for detection of APC2 at 1 - 2 μg/ml. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody (in aliquots) at -20°C.
Expiry Date 12 months
Background publications Zachariae, Shevchenko, Andrews et al.: "Mass spectrometric analysis of the anaphase-promoting complex from yeast: identification of a subunit related to cullins." in: Science (New York, N.Y.), Vol. 279, Issue 5354, pp. 1216-9, 1998 (PubMed).

Yu, Peters, King et al.: "Identification of a cullin homology region in a subunit of the anaphase-promoting complex." in: Science (New York, N.Y.), Vol. 279, Issue 5354, pp. 1219-22, 1998 (PubMed).

Jörgensen, Gräslund, Betz et al.: "Characterisation of the human APC1, the largest subunit of the anaphase-promoting complex." in: Gene, Vol. 262, Issue 1-2, pp. 51-9, 2001 (PubMed).

Peters: "The anaphase promoting complex/cyclosome: a machine designed to destroy." in: Nature reviews. Molecular cell biology, Vol. 7, Issue 9, pp. 644-56, 2006 (PubMed).

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