APH1A antibody (Middle Region)

Catalog No. ABIN499309

This Rabbit Polyclonal antibody specifically detects APH1A in WB, IHC (p) and EIA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for APH1A antibody (Middle Region) (ABIN499309)

Target: APH1A (Anterior Pharynx Defective 1 Homolog A (C. Elegans) (APH1A))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APH1A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-APH1A Antibody

Binding Specificity: Middle Region

Specificity: This antibody reacts to APH1.

Purification: Affinity chromatography purified via peptide column

Immunogen: APH1 antibody was raised against a 18 amino acid peptide from near the center of human APH1.

Isotype: IgG

Application Details

Application Notes: ELISA. Western Blot: APH1 antibody can be used for detection of APH1 at 0.5 - 1 μg/mL. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for APH1A

Target: APH1A (Anterior Pharynx Defective 1 Homolog A (C. Elegans) (APH1A))

Alternative Name: APH1A

Background: APH1 was initially identified as a component of the Notch pathway in C. elegans. Along with nicastrin, PEN2, and presenilin-1 APH1 is an essential component of the γsecretase complex which cleave the amyloid precursor protein (APP) at what are known as the gamma and gamma sites and can lead to the accumulation of the Amyloid beta peptide A beta cleavage product that is associated with Alzheimer's disease. APH1 exists in at least three distinct isoforms with APH1a as the principal isoform present in the gamma secretase complex. Mice deficient in this isoform, but not the other two, were lethal at E10.5, with impaired vascular and neural development observed. Despite its predicted molecular weight, APH1 protein often migrates at aberrant locations in SDS-PAGE.Synonyms: APH-1A, Aph-1 alpha, CGI-78, Gamma-secretase subunit APH-1A, PSF, Presenilin-stabilization factor

Gene ID: 51107

UniProt: Q96BI3

Pathways: Notch Signaling, Neurotrophin Signaling Pathway